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PDBsum entry 3rld

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protein ligands metals links
Lyase PDB id
3rld
Jmol
Contents
Protein chain
257 a.a.
Ligands
SO4 ×3
Metals
_ZN
Waters ×186
PDB id:
3rld
Name: Lyase
Title: Crystal structure of the y7i mutant of human carbonic anhydr
Structure: Carbonic anhydrase 2. Chain: a. Synonym: carbonate dehydratase ii, carbonic anhydrasE C, ca carbonic anhydrase ii, ca-ii. Engineered: yes. Mutation: yes
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: ca2. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.50Å     R-factor:   0.166     R-free:   0.211
Authors: B.S.Avvaru,R.Mikulski,R.Mckenna
Key ref: R.Mikulski et al. (2011). Kinetic and crystallographic studies of the role of tyrosine 7 in the active site of human carbonic anhydrase II. Arch Biochem Biophys, 506, 181-187. PubMed id: 21145876 DOI: 10.1016/j.abb.2010.12.004
Date:
19-Apr-11     Release date:   08-Feb-12    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00918  (CAH2_HUMAN) -  Carbonic anhydrase 2
Seq:
Struc:
260 a.a.
257 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.4.2.1.1  - Carbonate dehydratase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: H2CO3 = CO2 + H2O
H(2)CO(3)
= CO(2)
+ H(2)O
      Cofactor: Zn(2+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular space   11 terms 
  Biological process     angiotensin-mediated signaling pathway   21 terms 
  Biochemical function     protein binding     5 terms  

 

 
    Added reference    
 
 
DOI no: 10.1016/j.abb.2010.12.004 Arch Biochem Biophys 506:181-187 (2011)
PubMed id: 21145876  
 
 
Kinetic and crystallographic studies of the role of tyrosine 7 in the active site of human carbonic anhydrase II.
R.Mikulski, B.S.Avvaru, C.Tu, N.Case, R.McKenna, D.N.Silverman.
 
  ABSTRACT  
 
No abstract given.