PDBsum entry 3rdz

Hydrolase/hydrolase inhibitor

PDB id: 3rdz

Contents

Protein chains

220 a.a.

67 a.a.

Metals

_CA ×2

Waters ×300

PDB id:

3rdz

Name:

Hydrolase/hydrolase inhibitor

Title:

Crystal structure of rbti-trypsin complex at 2.26 angstrom resolution

Structure:

Cationic trypsin. Chain: a, b. Synonym: beta-trypsin, alpha-trypsin chain 1, alpha-trypsin chain 2. Bwi-1=protease inhibitor/trypsin inhibitor. Chain: c, d. Synonym: proteinase inhibitor. Engineered: yes

Source:

Bos taurus. Bovine. Organism_taxid: 9913. Other_details: purchased from applichem. Fagopyrum esculentum. Buckwheat. Organism_taxid: 3617. Gene: bwi-1, edn1. Expressed in: escherichia coli.

Resolution:

2.26Å R-factor: 0.183 R-free: 0.227

Authors:

L.F.Wang,M.Li,W.R.Chang

Key ref:

L.Wang et al. (2011). Conformational changes of rBTI from buckwheat upon binding to trypsin: implications for the role of the P(8)' residue in the potato inhibitor I family. Plos One, 6, e20950. PubMed id: 21698291

Date:

02-Apr-11 Release date: 06-Jul-11

Protein chains

P00760  (TRY1_BOVIN) -  Serine protease 1 from Bos taurus

Seq: 246 a.a.

Struc: 220 a.a.

Protein chains

Q9S9F3  (Q9S9F3_FAGES) -  Proteinase inhibitor from Fagopyrum esculentum

Seq: 69 a.a.

Struc: 67 a.a.

Enzyme reactions

• Enzyme class: Chains A, B: E.C.3.4.21.4 - trypsin.
• Reaction: Preferential cleavage: Arg-|-Xaa, Lys-|-Xaa.

Plos One 6:e20950 (2011)

PubMed id: 21698291

Conformational changes of rBTI from buckwheat upon binding to trypsin: implications for the role of the P(8)' residue in the potato inhibitor I family.

L.Wang, F.Zhao, M.Li, H.Zhang, Y.Gao, P.Cao, X.Pan, Z.Wang, W.Chang.

ABSTRACT

No abstract given.