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PDBsum entry 3qyz

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protein ligands links
Transferase/transferase inhibitor PDB id
3qyz
Jmol
Contents
Protein chain
341 a.a.
Ligands
Z8B
DMS ×11
SO4 ×2
BME
Waters ×458
PDB id:
3qyz
Name: Transferase/transferase inhibitor
Title: Crystal structure of erk2 in complex with an inhibitor
Structure: Mitogen-activated protein kinase 1. Chain: a. Synonym: map kinase 1, mapk 1, ert1, extracellular signal-r kinase 2, erk-2, map kinase isoform p42, p42-mapk, mitogen- protein kinase 2, map kinase 2, mapk 2. Engineered: yes
Source: Rattus norvegicus. Brown rat,rat,rats. Organism_taxid: 10116. Gene: mapk1, erk2, mapk, prkm1. Expressed in: escherichia coli. Expression_system_taxid: 562
Resolution:
1.46Å     R-factor:   0.163     R-free:   0.195
Authors: M.Gelin,S.Pochet,F.Hoh,M.Pirochi,J.-F.Guichou,J.-L.Ferrer,G.
Key ref: A.le Maire et al. (2011). In-plate protein crystallization, in situ ligand soaking and X-ray diffraction. Acta Crystallogr D Biol Crystallogr, 67, 747-755. PubMed id: 21904027 DOI: 10.1107/S0907444911023249
Date:
04-Mar-11     Release date:   24-Aug-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P63086  (MK01_RAT) -  Mitogen-activated protein kinase 1
Seq:
Struc:
358 a.a.
341 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.2.7.11.24  - Mitogen-activated protein kinase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + a protein = ADP + a phosphoprotein
ATP
+ protein
=
ADP
Bound ligand (Het Group name = Z8B)
matches with 58.06% similarity
+ phosphoprotein
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     mitotic spindle   21 terms 
  Biological process     intracellular signal transduction   40 terms 
  Biochemical function     nucleotide binding     15 terms  

 

 
    reference    
 
 
DOI no: 10.1107/S0907444911023249 Acta Crystallogr D Biol Crystallogr 67:747-755 (2011)
PubMed id: 21904027  
 
 
In-plate protein crystallization, in situ ligand soaking and X-ray diffraction.
A.le Maire, M.Gelin, S.Pochet, F.Hoh, M.Pirocchi, J.F.Guichou, J.L.Ferrer, G.Labesse.
 
  ABSTRACT  
 
No abstract given.