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PDBsum entry 3qsk

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protein Protein-protein interface(s) links
Hydrolase/immune system PDB id
3qsk
Jmol
Contents
Protein chains
119 a.a.
123 a.a.
Waters ×182
PDB id:
3qsk
Name: Hydrolase/immune system
Title: 5 histidine variant of the anti-rnase a vhh in complex with
Structure: Ribonuclease pancreatic. Chain: a. Synonym: rnase 1, rnase a. Engineered 5 histidine anti-rnase a camelid vhh a domain variant. Chain: b. Engineered: yes
Source: Bos taurus. Bovine,cow,domestic cattle,domestic cow. Organism_taxid: 9913. Camelus dromedarius. Arabian camel. Organism_taxid: 9838. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.75Å     R-factor:   0.187     R-free:   0.246
Authors: M.L.Murtaugh,S.W.Fanning,T.M.Sharma,A.M.Terry,J.R.Horn
Key ref: M.L.Murtaugh et al. (2011). A combinatorial histidine scanning library approach to engineer highly pH-dependent protein switches. Protein Sci, 20, 1619-1631. PubMed id: 21766385
Date:
21-Feb-11     Release date:   10-Aug-11    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P61823  (RNAS1_BOVIN) -  Ribonuclease pancreatic
Seq:
Struc:
150 a.a.
119 a.a.
Protein chain
No UniProt id for this chain
Struc: 123 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class: Chain A: E.C.3.1.27.5  - Pancreatic ribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     metabolic process   3 terms 
  Biochemical function     nucleic acid binding     6 terms  

 

 
Protein Sci 20:1619-1631 (2011)
PubMed id: 21766385  
 
 
A combinatorial histidine scanning library approach to engineer highly pH-dependent protein switches.
M.L.Murtaugh, S.W.Fanning, T.M.Sharma, A.M.Terry, J.R.Horn.
 
  ABSTRACT  
 
No abstract given.