PDBsum entry 3pyc

Lipid binding protein

PDB id: 3pyc

Contents

Protein chain

132 a.a.

Ligands

SO4 ×2

Metals

_CL

Waters ×54

PDB id:

3pyc

Name:

Lipid binding protein

Title:

Crystal structure of human smurf1 c2 domain

Structure:

E3 ubiquitin-protein ligase smurf1. Chain: a. Fragment: c2 domain, unp residues 13-140. Synonym: hsmurf1, smad ubiquitination regulatory factor 1, smad- specific e3 ubiquitin-protein ligase 1. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Gene: smurf1, kiaa1625. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.96Å R-factor: 0.206 R-free: 0.241

Authors:

X.Li,P.Li

Key ref:

X.Li and p.li Crystal structure of human smurf1 c2 domain. To be published, .

Date:

13-Dec-10 Release date: 06-Apr-11

Protein chain

Q9HCE7  (SMUF1_HUMAN) -  E3 ubiquitin-protein ligase SMURF1 from Homo sapiens

Seq: 757 a.a.

Struc: 132 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine