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PDBsum entry 3px7

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protein dna_rna ligands metals links
Isomerase/DNA PDB id
3px7
Jmol
Contents
Protein chain
552 a.a.
DNA/RNA
Ligands
_DT-_DT
Metals
_HG ×3
Waters ×140
PDB id:
3px7
Name: Isomerase/DNA
Title: Crystal structure of covalent complex of topoisomerase 1a wi substrate
Structure: DNA topoisomerase. Chain: a. Fragment: residues 1-595. Engineered: yes. Mutation: yes. DNA 5'-d( Tp Tp Gp Gp G)-3'. Chain: b. Engineered: yes. DNA 5'-d( A Ap Tp Gp Cp Gp Cp T)-3'.
Source: Escherichia coli dh1. Organism_taxid: 536056. Strain: atcc 33849 / dsm 4235 / ncib 12045 / k12 / dh1. Gene: 415337, ecdh1_2375. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Synthetic: yes
Resolution:
2.30Å     R-factor:   0.230     R-free:   0.269
Authors: Z.Zhang,Y.C.Tse-Dinh,B.Cheng
Key ref: Y.C.Tse-Dinh et al. Structure of e. Coli topoisomerase i trapped as coval complex intermediate with cleaved DNA. To be published, .
Date:
09-Dec-10     Release date:   20-Apr-11    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
P06612  (TOP1_ECOLI) -  DNA topoisomerase 1
Seq:
Struc:
 
Seq:
Struc:
865 a.a.
552 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.5.99.1.2  - Dna topoisomerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     DNA topological change   1 term 
  Biochemical function     DNA binding     3 terms