spacer
spacer

PDBsum entry 3pwt

Go to PDB code: 
protein ligands links
Isomerase PDB id
3pwt
Jmol
Contents
Protein chain
562 a.a.
Ligands
SO4 ×3
Waters ×421
PDB id:
3pwt
Name: Isomerase
Title: Crystal structure of mutant e.Coli topoisomerase ia
Structure: DNA topoisomerase. Chain: a. Fragment: n-terminal catalytical domain, 67k. Engineered: yes. Mutation: yes
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: topa, supx, b1274, jw1266. Expressed in: escherichia coli. Expression_system_taxid: 511693.
Resolution:
1.90Å     R-factor:   0.217     R-free:   0.249
Authors: Z.Zhang,C.Bokun,Y.-C.Tse-Dinh
Key ref: Z.Zhang et al. Crystal structure of mutant e.Coli topoisomerase ia. To be published, .
Date:
08-Dec-10     Release date:   29-Dec-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P06612  (TOP1_ECOLI) -  DNA topoisomerase 1
Seq:
Struc:
 
Seq:
Struc:
865 a.a.
562 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.5.99.1.2  - Dna topoisomerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     DNA topological change   1 term 
  Biochemical function     DNA binding     3 terms