spacer
spacer

PDBsum entry 3pq3

Go to PDB code: 
protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
3pq3
Jmol
Contents
Protein chains
726 a.a. *
Ligands
HDD-HDE ×4
H2S ×4
Waters ×3442
* Residue conservation analysis
PDB id:
3pq3
Name: Oxidoreductase
Title: Structure of i274c variant of e. Coli kate[] - images 7-12
Structure: Catalase hpii. Chain: a, b, c, d. Synonym: hydroxyperoxidase ii. Engineered: yes. Mutation: yes
Source: Escherichia coli. Organism_taxid: 83333. Gene: b1732, jw1721, kate. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.79Å     R-factor:   0.145     R-free:   0.189
Authors: P.C.Loewen,V.Jha,S.Louis,P.Chelikani,X.Carpena,I.Fita
Key ref: V.Jha et al. (2011). Modulation of heme orientation and binding by a single residue in catalase HPII of Escherichia coli. Biochemistry, 50, 2101-2110. PubMed id: 21332158 DOI: 10.1021/bi200027v
Date:
25-Nov-10     Release date:   22-Dec-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P21179  (CATE_ECOLI) -  Catalase HPII
Seq:
Struc:
 
Seq:
Struc:
753 a.a.
726 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.1.11.1.6  - Catalase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: 2 H2O2 = O2 + 2 H2O
2 × H(2)O(2)
= O(2)
+ 2 × H(2)O
      Cofactor: Heme; Mn(2+)
Heme
Bound ligand (Het Group name = HDD) matches with 93.33% similarity
Mn(2+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   2 terms 
  Biological process     oxidation-reduction process   5 terms 
  Biochemical function     oxidoreductase activity     7 terms  

 

 
    reference    
 
 
DOI no: 10.1021/bi200027v Biochemistry 50:2101-2110 (2011)
PubMed id: 21332158  
 
 
Modulation of heme orientation and binding by a single residue in catalase HPII of Escherichia coli.
V.Jha, S.Louis, P.Chelikani, X.Carpena, L.J.Donald, I.Fita, P.C.Loewen.
 
  ABSTRACT  
 
No abstract given.