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PDBsum entry 3pjd

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protein ligands Protein-protein interface(s) links
Oxidoreductase/oxidoreductase inhibitor PDB id
3pjd
Jmol
Contents
Protein chains
257 a.a.
Ligands
NAD ×2
TCL ×2
Waters ×84
PDB id:
3pjd
Name: Oxidoreductase/oxidoreductase inhibitor
Title: Structure of enr g93a mutant-NAD+-triclosan complex
Structure: Enoyl-[acyl-carrier-protein] reductase [nadh]. Chain: a, b. Synonym: nadh-dependent enoyl-acp reductase. Engineered: yes. Mutation: yes
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: envm, fabi. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.50Å     R-factor:   0.210     R-free:   0.234
Authors: H.T.Kim,D.G.Shin,H.J.Chang
Key ref: N.J.Singh et al. (2011). Structural basis of triclosan resistance. J Struct Biol, 174, 173-179. PubMed id: 21094257
Date:
10-Nov-10     Release date:   20-Apr-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P0AEK4  (FABI_ECOLI) -  Enoyl-[acyl-carrier-protein] reductase [NADH] FabI
Seq:
Struc:
262 a.a.
257 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.1.3.1.9  - Enoyl-[acyl-carrier-protein] reductase (NADH).
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: An acyl-[acyl-carrier protein] + NAD+ = a trans-2,3-dehydroacyl-[acyl- carrier protein] + NADH
acyl-[acyl-carrier protein]
+
NAD(+)
Bound ligand (Het Group name = NAD)
corresponds exactly
= trans-2,3-dehydroacyl-[acyl- carrier protein]
+ NADH
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   1 term 
  Biological process     oxidation-reduction process   9 terms 
  Biochemical function     oxidoreductase activity     3 terms  

 

 
    reference    
 
 
J Struct Biol 174:173-179 (2011)
PubMed id: 21094257  
 
 
Structural basis of triclosan resistance.
N.J.Singh, D.Shin, H.M.Lee, H.T.Kim, H.J.Chang, J.M.Cho, K.S.Kim, S.Ro.
 
  ABSTRACT  
 
No abstract given.