PDBsum entry 3phc

Transferase/transferase inhibitor

PDB id: 3phc

Contents

Protein chains

(+ 0 more) 243 a.a.

Ligands

PO4 ×6

IM5 ×6

Metals

__K ×3

Waters ×312

PDB id:

3phc

Name:

Transferase/transferase inhibitor

Title:

Crystal structure of plasmodium falciparum purine nucleoside phosphorylase in complex with dadme-immg

Structure:

Purine nucleoside phosphorylase. Chain: a, b, c, d, e, f. Engineered: yes

Source:

Plasmodium falciparum. Organism_taxid: 5833. Gene: pnp. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

2.00Å R-factor: 0.200 R-free: 0.235

Authors:

M.Ho,A.A.Edwards,S.C.Almo,V.L.Schramm

Key ref:

M.Ho et al. Crystal structure of plasmodium falciparum purine nuc phosphorylase in complex with dadme-Immg. To be published, .

Date:

03-Nov-10 Release date: 09-Nov-11

Protein chains

Q8I3X4  (Q8I3X4_PLAF7) -  Purine nucleoside phosphorylase from Plasmodium falciparum (isolate 3D7)

Seq: 245 a.a.

Struc: 243 a.a.

Enzyme reactions

• Enzyme class 2: E.C.2.4.2.1 - purine-nucleoside phosphorylase.
• Reaction:
  1. a purine D-ribonucleoside + phosphate = a purine nucleobase + alpha- D-ribose 1-phosphate
  2. a purine 2'-deoxy-D-ribonucleoside + phosphate = a purine nucleobase + 2-deoxy-alpha-D-ribose 1-phosphate

purine D-ribonucleoside + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly) = purine nucleobase + alpha- D-ribose 1-phosphate

purine 2'-deoxy-D-ribonucleoside + phosphate (Bound ligand (Het Group name = PO4) corresponds exactly) = purine nucleobase + 2-deoxy-alpha-D-ribose 1-phosphate

• Enzyme class 3: E.C.2.4.2.44 - S-methyl-5'-thioinosine phosphorylase.
• Reaction: S-methyl-5'-thioinosine + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate + hypoxanthine

S-methyl-5'-thioinosine (Bound ligand (Het Group name = PO4) corresponds exactly) + phosphate = 5-(methylsulfanyl)-alpha-D-ribose 1-phosphate (Bound ligand (Het Group name = IM5) matches with 42.86% similarity) + hypoxanthine

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site