spacer
spacer

PDBsum entry 3oqy

Go to PDB code: 
protein Protein-protein interface(s) links
Hydrolase PDB id
3oqy
Jmol
Contents
Protein chains
15 a.a.
103 a.a. *
14 a.a. *
Waters ×354
* Residue conservation analysis
PDB id:
3oqy
Name: Hydrolase
Title: Semi-synthetic ribonuclease s: para-cyano-phenylalanine at p
Structure: Ribonuclease pancreatic. Chain: b, a. Fragment: unp residues 27-41. Synonym: rnase 1, rnase a. Engineered: yes. Ribonuclease pancreatic. Chain: b, a. Fragment: unp residues 47-150. Synonym: rnase 1, rnase a.
Source: Synthetic: yes. Bos taurus. Bovine,cow,domestic cattle,domestic cow. Organism_taxid: 9913. Other_details: made by solid phase peptide synthesis. Other_details: subtilisin treated rnase a
Resolution:
1.49Å     R-factor:   0.203     R-free:   0.222
Authors: A.T.Fafarman,S.G.Boxer
Key ref: A.T.Fafarman and S.G.Boxer (2010). Nitrile bonds as infrared probes of electrostatics in ribonuclease S. J Phys Chem B, 114, 13536-13544. PubMed id: 20883003 DOI: 10.1021/jp106406p
Date:
04-Sep-10     Release date:   20-Oct-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P61823  (RNAS1_BOVIN) -  Ribonuclease pancreatic
Seq:
Struc:
150 a.a.
15 a.a.*
Protein chains
Pfam   ArchSchema ?
P61823  (RNAS1_BOVIN) -  Ribonuclease pancreatic
Seq:
Struc:
150 a.a.
103 a.a.
Protein chain
Pfam   ArchSchema ?
P61823  (RNAS1_BOVIN) -  Ribonuclease pancreatic
Seq:
Struc:
150 a.a.
14 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains b, B, a, A: E.C.3.1.27.5  - Pancreatic ribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biochemical function     nucleic acid binding     2 terms  

 

 
DOI no: 10.1021/jp106406p J Phys Chem B 114:13536-13544 (2010)
PubMed id: 20883003  
 
 
Nitrile bonds as infrared probes of electrostatics in ribonuclease S.
A.T.Fafarman, S.G.Boxer.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21336362 X.S.Gai, B.A.Coutifaris, S.H.Brewer, and E.E.Fenlon (2011).
A direct comparison of azide and nitrile vibrational probes.
  Phys Chem Chem Phys, 13, 5926-5930.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.