PDBsum entry 3olm

Ligase

PDB id: 3olm

Contents

Protein chains

412 a.a.

74 a.a.

Waters ×51

PDB id:

3olm

Name:

Ligase

Title:

Structure and function of a ubiquitin binding site within the catalytic domain of a hect ubiquitin ligase

Structure:

E3 ubiquitin-protein ligase rsp5. Chain: a. Fragment: ww3 and hect domain. Synonym: reverses spt-phenotype protein 5. Engineered: yes. Ubiquitin. Chain: d. Engineered: yes

Source:

Saccharomyces cerevisiae. Yeast. Organism_taxid: 4932. Gene: rsp5, mdp1, npi1, yer125w, sygp-orf41. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: ubi4, scd2, yll039c. Expression_system_taxid: 562

Resolution:

2.50Å R-factor: 0.223 R-free: 0.272

Authors:

H.C.Kim,A.Steffen,J.Chen,J.M.Huibregtse

Key ref:

H.C.Kim et al. (2011). Structure and function of a HECT domain ubiquitin-binding site. Embo Rep, 12, 334-341. PubMed id: 21399621

Date:

26-Aug-10 Release date: 23-Mar-11

Protein chain

P39940  (RSP5_YEAST) -  E3 ubiquitin-protein ligase RSP5 from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 809 a.a.

Struc: 412 a.a.

Protein chain

P0CG63  (UBI4P_YEAST) -  Polyubiquitin from Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

Seq: 381 a.a.

Struc: 74 a.a.

Enzyme reactions

• Enzyme class 1: Chain A: E.C.2.3.2.26 - HECT-type E3 ubiquitin transferase.
• Reaction: S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N₆- ubiquitinyl-[acceptor protein]-L-lysine
• Enzyme class 2: Chain D: E.C.?

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Embo Rep 12:334-341 (2011)

PubMed id: 21399621

Structure and function of a HECT domain ubiquitin-binding site.

H.C.Kim, A.M.Steffen, M.L.Oldham, J.Chen, J.M.Huibregtse.

ABSTRACT

No abstract given.