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PDBsum entry 3olb

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protein dna_rna ligands metals Protein-protein interface(s) links
Transferase/RNA PDB id
3olb
Jmol
Contents
Protein chains
461 a.a. *
DNA/RNA
Ligands
DCT ×4
IPA ×12
Metals
_ZN ×4
Waters ×898
* Residue conservation analysis
PDB id:
3olb
Name: Transferase/RNA
Title: Poliovirus polymerase elongation complex with 2',3'-dideoxy-
Structure: Polymerase. Chain: a, e, i, m. Fragment: unp residues 1749-2209. Engineered: yes. Mutation: yes. RNA (5'- r( Ap Ap Gp Up Cp U Cp Cp Ap Gp Gp Up Cp Up Cp Up Cp Gp Up P Ap Ap A)-3'). Chain: b, f, j, n.
Source: Human poliovirus 1. Organism_taxid: 12080. Strain: mahoney. Gene: 3d. Expressed in: escherichia coli. Expression_system_taxid: 469008. Synthetic: yes. Other_details: RNA prepared by t7 RNA polymerase transcript followed by glms ribozyme self-cleavage.
Resolution:
2.41Å     R-factor:   0.220     R-free:   0.261
Authors: P.Gong,O.B.Peersen
Key ref: P.Gong and O.B.Peersen (2010). Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase. Proc Natl Acad Sci U S A, 107, 22505-22510. PubMed id: 21148772
Date:
25-Aug-10     Release date:   22-Dec-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
B3VQP5  (B3VQP5_9ENTO) -  Polyprotein
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
2209 a.a.
461 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     transcription, DNA-dependent   2 terms 
  Biochemical function     RNA binding     2 terms  

 

 
Proc Natl Acad Sci U S A 107:22505-22510 (2010)
PubMed id: 21148772  
 
 
Structural basis for active site closure by the poliovirus RNA-dependent RNA polymerase.
P.Gong, O.B.Peersen.
 
  ABSTRACT  
 
No abstract given.