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PDBsum entry 3oeh

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protein ligands metals Protein-protein interface(s) links
Hydrolase PDB id
3oeh
Jmol
Contents
Protein chains
(+ 3 more) 482 a.a. *
(+ 3 more) 470 a.a. *
266 a.a. *
116 a.a. *
49 a.a. *
246 a.a. *
84 a.a. *
34 a.a. *
201 a.a. *
17 a.a. *
27 a.a. *
Ligands
ANP ×15
Metals
_MG ×15
* Residue conservation analysis
PDB id:
3oeh
Name: Hydrolase
Title: Structure of four mutant forms of yeast f1 atpase: beta-v279
Structure: Atp synthase subunit alpha. Chain: a, b, c, j, k, l, s, t, u. Fragment: unp residues 36-545. Engineered: yes. Atp synthase subunit beta. Chain: d, e, f, m, n, o, v, w, x. Fragment: unp residues 34-511. Engineered: yes. Mutation: yes.
Source: Saccharomyces cerevisiae. Brewer's yeast,lager beer yeast,yeast. Organism_taxid: 4932. Gene: atp1, ybl099w, ybl0827. Expressed in: saccharomyces cerevisiae. Expression_system_taxid: 4932. Gene: atp2, yjr121w, j2041. Gene: atp3, atp3a, atp3b, ybr039w, ybr0408. Gene: atp16, ydl004w, yd8119.03, d2935.
Resolution:
3.00Å     R-factor:   0.210     R-free:   0.270
Authors: D.Arsenieva,J.Symersky,Y.Wang,V.Pagadala,D.M.Mueller
Key ref: D.Arsenieva et al. (2010). Crystal structures of mutant forms of the yeast F1 ATPase reveal two modes of uncoupling. J Biol Chem, 285, 36561-36569. PubMed id: 20843806 DOI: 10.1074/jbc.M110.174383
Date:
12-Aug-10     Release date:   15-Sep-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P07251  (ATPA_YEAST) -  ATP synthase subunit alpha, mitochondrial
Seq:
Struc:
 
Seq:
Struc:
545 a.a.
482 a.a.
Protein chains
Pfam   ArchSchema ?
P00830  (ATPB_YEAST) -  ATP synthase subunit beta, mitochondrial
Seq:
Struc:
511 a.a.
470 a.a.*
Protein chain
Pfam   ArchSchema ?
P38077  (ATPG_YEAST) -  ATP synthase subunit gamma, mitochondrial
Seq:
Struc:
311 a.a.
266 a.a.
Protein chain
Pfam   ArchSchema ?
Q12165  (ATPD_YEAST) -  ATP synthase subunit delta, mitochondrial
Seq:
Struc:
160 a.a.
116 a.a.
Protein chain
Pfam   ArchSchema ?
P21306  (ATP5E_YEAST) -  ATP synthase subunit epsilon, mitochondrial
Seq:
Struc:
62 a.a.
49 a.a.
Protein chain
Pfam   ArchSchema ?
P00830  (ATPB_YEAST) -  ATP synthase subunit beta, mitochondrial
Seq:
Struc:
 
Seq:
Struc:
511 a.a.
246 a.a.*
Protein chain
Pfam   ArchSchema ?
P38077  (ATPG_YEAST) -  ATP synthase subunit gamma, mitochondrial
Seq:
Struc:
311 a.a.
246 a.a.
Protein chain
Pfam   ArchSchema ?
Q12165  (ATPD_YEAST) -  ATP synthase subunit delta, mitochondrial
Seq:
Struc:
160 a.a.
84 a.a.
Protein chain
Pfam   ArchSchema ?
P21306  (ATP5E_YEAST) -  ATP synthase subunit epsilon, mitochondrial
Seq:
Struc:
62 a.a.
34 a.a.
Protein chain
Pfam   ArchSchema ?
P38077  (ATPG_YEAST) -  ATP synthase subunit gamma, mitochondrial
Seq:
Struc:
311 a.a.
201 a.a.
Protein chain
Pfam   ArchSchema ?
Q12165  (ATPD_YEAST) -  ATP synthase subunit delta, mitochondrial
Seq:
Struc:
160 a.a.
17 a.a.
Protein chain
Pfam   ArchSchema ?
P21306  (ATP5E_YEAST) -  ATP synthase subunit epsilon, mitochondrial
Seq:
Struc:
62 a.a.
27 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 209 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains D, E, F, N, P, V, W, X: E.C.3.6.3.14  - H(+)-transporting two-sector ATPase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: ATP + H2O + H+(In) = ADP + phosphate + H+(Out)
ATP
+ H(2)O
+ H(+)(In)
=
ADP
Bound ligand (Het Group name = ANP)
matches with 81.00% similarity
+ phosphate
+ H(+)(Out)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   11 terms 
  Biological process     transport   8 terms 
  Biochemical function     hydrolase activity     8 terms  

 

 
    reference    
 
 
DOI no: 10.1074/jbc.M110.174383 J Biol Chem 285:36561-36569 (2010)
PubMed id: 20843806  
 
 
Crystal structures of mutant forms of the yeast F1 ATPase reveal two modes of uncoupling.
D.Arsenieva, J.Symersky, Y.Wang, V.Pagadala, D.M.Mueller.
 
  ABSTRACT  
 
No abstract given.