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PDBsum entry 3nss

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protein ligands metals Protein-protein interface(s) links
Hydrolase PDB id
3nss
Jmol
Contents
Protein chains
387 a.a.
Ligands
NAG-NAG-MAN-MAN
NAG ×3
ACT ×4
Metals
_CA ×5
Waters ×889
PDB id:
3nss
Name: Hydrolase
Title: The 2009 pandemic h1n1 neuraminidase n1 lacks the 150-cavity active sites
Structure: Neuraminidase. Chain: a, b. Fragment: unp residues 82-469. Engineered: yes
Source: Influenza a virus. Organism_taxid: 641501. Strain: a/california/04/2009 h1n1. Gene: na. Expressed in: spodoptera frugiperda. Expression_system_taxid: 7108. Expression_system_cell_line: sf9.
Resolution:
1.90Å     R-factor:   0.171     R-free:   0.196
Authors: Q.Li,J.X.Qi,W.Zhang,C.J.Vavricka,Y.Shi,G.F.Gao
Key ref: Q.Li et al. (2010). The 2009 pandemic H1N1 neuraminidase N1 lacks the 150-cavity in its active site. Nat Struct Mol Biol, 17, 1266-1268. PubMed id: 20852645
Date:
02-Jul-10     Release date:   22-Sep-10    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
C3W5S3  (C3W5S3_I09A0) -  Neuraminidase
Seq:
Struc:
469 a.a.
387 a.a.
Key:    PfamA domain  Secondary structure  CATH domain

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   3 terms 
  Biological process     carbohydrate metabolic process   1 term 
  Biochemical function     exo-alpha-sialidase activity     1 term  

 

 
Nat Struct Mol Biol 17:1266-1268 (2010)
PubMed id: 20852645  
 
 
The 2009 pandemic H1N1 neuraminidase N1 lacks the 150-cavity in its active site.
Q.Li, J.Qi, W.Zhang, C.J.Vavricka, Y.Shi, J.Wei, E.Feng, J.Shen, J.Chen, D.Liu, J.He, J.Yan, H.Liu, H.Jiang, M.Teng, X.Li, G.F.Gao.
 
  ABSTRACT  
 
Influenza A virus neuraminidase can be classified into groups 1 and 2 on the basis of its primary structure. The main structural feature of group 1 neuraminidase is an extra cavity in the active site, the 150-cavity. Here we present the crystal structure of neuraminidase from the 2009 pandemic H1N1 influenza strain. In contrast to other characterized N1 neuraminidases, which are all members of group 1, 2009 H1N1 neuraminidase does not have a 150-cavity.