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PDBsum entry 3npl

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protein ligands Protein-protein interface(s) links
Oxidoreductase PDB id
3npl
Jmol
Contents
Protein chains
456 a.a. *
Ligands
HEM ×2
SO4
RU8
Waters ×436
* Residue conservation analysis
PDB id:
3npl
Name: Oxidoreductase
Title: Structure of ru(bpy)2(a-phen)(k97c) p450 bm3 heme domain, a modified p450 bm3 mutant
Structure: Bifunctional p-450/NADPH-p450 reductase. Chain: a, b. Fragment: unp residues 1-464. Synonym: cytochrome p450(bm-3), cytochrome p450bm-3, cytoch 102, NADPH--cytochrome p450 reductase. Engineered: yes. Mutation: yes
Source: Bacillus megaterium. Organism_taxid: 1404. Gene: cyp102, cyp102a1. Expressed in: escherichia coli. Expression_system_taxid: 469008.
Resolution:
2.40Å     R-factor:   0.203     R-free:   0.238
Authors: M.Ener,Y.-T.Lee,D.B.Goodin,J.R.Winkler,H.B.Gray,L.Cheruzel
Key ref: M.Ener et al. Structure of ru(bpy)2(a-Phen)(k97c) p450 bm3 heme dom ruthenium modified p450 bm3 mutant. To be published, .
Date:
28-Jun-10     Release date:   25-Aug-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P14779  (CPXB_BACME) -  Bifunctional P-450/NADPH-P450 reductase
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1049 a.a.
456 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 3 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class 2: E.C.1.14.14.1  - Unspecific monooxygenase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: RH + reduced flavoprotein + O2 = ROH + oxidized flavoprotein + H2O
RH
+ reduced flavoprotein
+ O(2)
= ROH
+ oxidized flavoprotein
+ H(2)O
      Cofactor: Heme-thiolate
   Enzyme class 3: E.C.1.6.2.4  - NADPH--hemoprotein reductase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: NADPH + n oxidized hemoprotein = NADP+ + n reduced hemoprotein
NADPH
+ n oxidized hemoprotein
= NADP(+)
+ n reduced hemoprotein
      Cofactor: FAD; FMN
FAD
FMN
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     oxidation-reduction process   1 term 
  Biochemical function     oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen     3 terms