PDBsum entry 3nh0

Hydrolase/DNA

PDB id: 3nh0

Contents

Protein chains

206 a.a. *

DNA/RNA

Waters ×270

* Residue conservation analysis

PDB id:

3nh0

Name:

Hydrolase/DNA

Title:

Crystal structure of rnase t in complex with a non-preferred ssdna (aac)

Structure:

Ribonuclease t. Chain: a, b. Synonym: rnase t, exoribonuclease t. Engineered: yes. 5'-d( Tp Tp Ap Cp Ap Ap C)-3'. Chain: c, d. Engineered: yes. Other_details: ssdna

Source:

Escherichia coli. Organism_taxid: 562. Strain: jm109 / atcc 53323. Gene: rnt. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes

Resolution:

2.30Å R-factor: 0.200 R-free: 0.249

Authors:

Y.-Y.Hsiao,H.S.Yuan

Key ref:

Y.Y.Hsiao et al. (2011). Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation. Nat Chem Biol, 7, 236-243. PubMed id: 21317904

Date:

14-Jun-10 Release date: 16-Feb-11

Protein chains

P30014  (RNT_ECOLI) -  Ribonuclease T from Escherichia coli (strain K12)

Seq: 215 a.a.

Struc: 206 a.a.

DNA/RNA chains

A-A-C 3 bases

A-A-C 3 bases

Enzyme reactions

• Enzyme class: E.C.3.1.13.- - ?????

Nat Chem Biol 7:236-243 (2011)

PubMed id: 21317904

Structural basis for RNA trimming by RNase T in stable RNA 3'-end maturation.

Y.Y.Hsiao, C.C.Yang, C.L.Lin, J.L.Lin, Y.Duh, H.S.Yuan.

ABSTRACT

No abstract given.