PDBsum entry 3n4j

Transferase

PDB id

3n4j

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Contents

			Protein chain

					161 a.a. *

			Ligands

					SO4 ×2

			Waters ×145

* Residue conservation analysis

PDB id:

3n4j

Links

Name:

Transferase

Title:

Putative RNA methyltransferase from yersinia pestis

Structure:

RNA methyltransferase. Chain: a. Engineered: yes

Source:

Yersinia pestis. Organism_taxid: 214092. Strain: co92. Gene: cspr, ypo0071, yp_0071. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.47Å

R-factor:

0.145

R-free:

0.186

Authors:

J.Osipiuk,N.Maltseva,S.Peterson,W.F.Anderson,A.Joachimiak,Center For Structural Genomics Of Infectious Diseases (Csgid)

Key ref:

J.Osipiuk et al. X-Ray crystal structure of putative RNA methyltransfe from yersinia pestis.. To be published, .

Date:

21-May-10

Release date:

23-Jun-10

PROCHECK

	Headers

	References

Protein chain

Q74Y93 (TRML_YERPE) - tRNA (cytidine(34)-2'-O)-methyltransferase from Yersinia pestis

Seq: Struc:					162 a.a. 161 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.2.1.1.207 - tRNA (cytidine(34)-2'-O)-methyltransferase.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

1.	cytidine₃₄ in tRNA + S-adenosyl-L-methionine = 2'-O- methylcytidine₃₄ in tRNA + S-adenosyl-L-homocysteine + H⁺
2.	5-carboxymethylaminomethyluridine₃₄ in tRNA(Leu) + S-adenosyl-L- methionine = 5-carboxymethylaminomethyl-2'-O-methyluridine₃₄ in tRNA(Leu) + S-adenosyl-L-homocysteine + H⁺

cytidine(34) in tRNA	+	S-adenosyl-L-methionine	=	2'-O- methylcytidine(34) in tRNA	+	S-adenosyl-L-homocysteine	+	H(+)

5-carboxymethylaminomethyluridine(34) in tRNA(Leu)	+	S-adenosyl-L- methionine	=	5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu)	+	S-adenosyl-L-homocysteine	+	H(+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site