PDBsum entry 3n2s

Oxidoreductase

PDB id: 3n2s

Contents

Protein chains

248 a.a. *

Ligands

FMN ×4

Metals

_CL ×2

Waters ×392

* Residue conservation analysis

PDB id:

3n2s

Name:

Oxidoreductase

Title:

Structure of nfra1 nitroreductase from b. Subtilis

Structure:

NADPH-dependent nitro/flavin reductase. Chain: a, b, c, d. Synonym: NADPH-dependent oxidoreductase, NADPH-dependent fmn reductase, NADPH-dependent nitroreductase. Engineered: yes

Source:

Bacillus subtilis. Organism_taxid: 1423. Strain: bacillus cncm i-1915. Gene: nfra1, nfra, ywcg, bsu38110, ipa-43d. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.95Å R-factor: 0.174 R-free: 0.198

Authors:

S.Morera,V.Gueguen-Chaignon,P.Meyer,S.Cortial,J.Ouazzani

Key ref:

S.Cortial et al. (2010). NADH oxidase activity of Bacillus subtilis nitroreductase NfrA1: insight into its biological role. Febs Lett, 584, 3916-3922. PubMed id: 20727352

Date:

19-May-10 Release date: 15-Sep-10

Protein chains

P39605  (NFRA1_BACSU) -  FMN reductase (NADPH) from Bacillus subtilis (strain 168)

Seq: 249 a.a.

Struc: 248 a.a.

Enzyme reactions

• Enzyme class: E.C.1.5.1.38 - Fmn reductase (NADPH).
• Reaction: FMNH2 + NADP⁺ = FMN + NADPH + 2 H⁺

FMNH2 (Bound ligand (Het Group name = FMN) corresponds exactly) + NADP(+) = FMN + NADPH + 2 × H(+)

• Cofactor: FMN

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

reference

Febs Lett 584:3916-3922 (2010)

PubMed id: 20727352

NADH oxidase activity of Bacillus subtilis nitroreductase NfrA1: insight into its biological role.

S.Cortial, P.Chaignon, B.I.Iorga, S.Aymerich, G.Truan, V.Gueguen-Chaignon, P.Meyer, S.Moréra, J.Ouazzani.

ABSTRACT

No abstract given.