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PDBsum entry 3mx1

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protein links
Hydrolase PDB id
3mx1
Jmol
Contents
Protein chain
208 a.a. *
Waters ×170
* Residue conservation analysis
PDB id:
3mx1
Name: Hydrolase
Title: The structure of giy-yig endonuclease r.Eco29ki
Structure: Eco29kir. Chain: a. Synonym: restriction endonuclease. Engineered: yes. Mutation: yes
Source: Escherichia coli. Organism_taxid: 562. Gene: eco29kir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.30Å     R-factor:   0.185     R-free:   0.228
Authors: A.N.S.Mak,A.R.Lambert,B.L.Stoddard
Key ref: A.N.Mak et al. (2010). Folding, DNA recognition, and function of GIY-YIG endonucleases: crystal structures of R.Eco29kI. Structure, 18, 1321-1331. PubMed id: 20800503
Date:
06-May-10     Release date:   08-Sep-10    
PROCHECK
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 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q46944  (Q46944_ECOLX) -  Eco29kIR
Seq:
Struc:
214 a.a.
208 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.1.21.4  - Type Ii site-specific deoxyribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
      Cofactor: Magnesium
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     metabolic process   3 terms 
  Biochemical function     hydrolase activity     3 terms  

 

 
Structure 18:1321-1331 (2010)
PubMed id: 20800503  
 
 
Folding, DNA recognition, and function of GIY-YIG endonucleases: crystal structures of R.Eco29kI.
A.N.Mak, A.R.Lambert, B.L.Stoddard.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21220111 B.L.Stoddard (2011).
Homing endonucleases: from microbial genetic invaders to reagents for targeted DNA modification.
  Structure, 19, 7.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.