PDBsum entry 3mfc

Hydrolase

PDB id: 3mfc

Contents

Protein chain

187 a.a. *

Ligands

SO4 ×2

Waters ×219

* Residue conservation analysis

PDB id:

3mfc

Name:

Hydrolase

Title:

Computationally designed end0-1,4-beta,xylanase

Structure:

Endo-1,4-beta-xylanase. Chain: a. Fragment: unp residues 44-235. Engineered: yes. Mutation: yes

Source:

Thermopolyspora flexuosa. Organism_taxid: 103836. Gene: xyn11a. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.70Å R-factor: 0.140 R-free: 0.189

Authors:

A.Morin,J.M.Harp

Key ref:

A.Morin et al. (2011). Computational design of an endo-1,4-beta-xylanase ligand binding site. Protein Eng Des Sel, 24, 503-516. PubMed id: 21349882

Date:

01-Apr-10 Release date: 10-Nov-10

Protein chain

Q8GMV7  (Q8GMV7_9ACTN) -  Endo-1,4-beta-xylanase from Thermopolyspora flexuosa

Seq: 344 a.a.

Struc: 187 a.a.*

* PDB and UniProt seqs differ at 8 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.3.2.1.8 - endo-1,4-beta-xylanase.
• Reaction: Endohydrolysis of 1,4-beta-D-xylosidic linkages in xylans.

Protein Eng Des Sel 24:503-516 (2011)

PubMed id: 21349882

Computational design of an endo-1,4-beta-xylanase ligand binding site.

A.Morin, K.W.Kaufmann, C.Fortenberry, J.M.Harp, L.S.Mizoue, J.Meiler.

ABSTRACT

No abstract given.