PDBsum entry 3luf

Signaling protein

PDB id: 3luf

Contents

Protein chains

244 a.a. *

Ligands

DMS ×3

Metals

_MG ×4

Waters ×169

* Residue conservation analysis

PDB id:

3luf

Name:

Signaling protein

Title:

Structure of probable two-component system response regulator/ggdef domain protein

Structure:

Two-component system response regulator/ggdef domain protein. Chain: a, b. Fragment: sequence database residues 2-249. Engineered: yes

Source:

Aeromonas salmonicida. Organism_taxid: 382245. Strain: a449. Gene: asa_2441. Expressed in: escherichia coli. Expression_system_taxid: 469008.

Resolution:

1.76Å R-factor: 0.189 R-free: 0.226

Authors:

U.A.Ramagopal,R.Toro,S.K.Burley,S.C.Almo,New York Sgx Research Center For Structural Genomics (Nysgxrc)

Key ref:

U.A.Ramagopal et al. Structure of probable two-Component system response regulator/ggdef domain protein. To be published, .

Date:

17-Feb-10 Release date: 02-Mar-10

Protein chains

A4SNL2  (A4SNL2_AERS4) -  diguanylate cyclase from Aeromonas salmonicida (strain A449)

Seq: 415 a.a.

Struc: 244 a.a.*

* PDB and UniProt seqs differ at 4 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.7.65 - diguanylate cyclase.
• Reaction: 2 GTP = 3',3'-c-di-GMP + 2 diphosphate
• Cofactor: Mg(2+); Mn(2+)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site