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PDBsum entry 3lu0

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protein metals Protein-protein interface(s) links
Transferase PDB id
3lu0
Jmol
Contents
Protein chains
235 a.a. *
1335 a.a. *
1364 a.a. *
89 a.a. *
Metals
_ZN ×2
_MG
* Residue conservation analysis
PDB id:
3lu0
Name: Transferase
Title: Molecular model of escherichia coli core RNA polymerase
Structure: DNA-directed RNA polymerase subunit alpha. Chain: a, b. Synonym: rnap subunit alpha, transcriptase subunit alpha, r polymerase subunit alpha. Engineered: yes. DNA-directed RNA polymerase subunit beta. Chain: c. Synonym: rnap subunit beta, transcriptase subunit beta, RNA polymerase subunit beta.
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b3295, jw3257, pez, phs, rpoa, sez. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: b3987, gron, jw3950, nitb, rif, ron, rpob, rpoc, stl, tabd. Gene: b3988, jw3951, rpoc, rpod, tabb.
Authors: S.A.Darst
Key ref: N.Opalka et al. (2010). Complete structural model of Escherichia coli RNA polymerase from a hybrid approach. PLoS Biol, 8, . PubMed id: 20856905
Date:
16-Feb-10     Release date:   29-Sep-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
P0A7Z4  (RPOA_ECOLI) -  DNA-directed RNA polymerase subunit alpha
Seq:
Struc:
329 a.a.
235 a.a.
Protein chain
Pfam   ArchSchema ?
P0A8V2  (RPOB_ECOLI) -  DNA-directed RNA polymerase subunit beta
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1342 a.a.
1335 a.a.*
Protein chain
Pfam   ArchSchema ?
P0A8T7  (RPOC_ECOLI) -  DNA-directed RNA polymerase subunit beta'
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1407 a.a.
1364 a.a.
Protein chain
Pfam   ArchSchema ?
P0A800  (RPOZ_ECOLI) -  DNA-directed RNA polymerase subunit omega
Seq:
Struc:
91 a.a.
89 a.a.
Key:    PfamA domain  Secondary structure
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: Chains A, B, C, D, E: E.C.2.7.7.6  - DNA-directed Rna polymerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Nucleoside triphosphate
+ RNA(n)
= diphosphate
+ RNA(n+1)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     membrane   4 terms 
  Biological process     protein complex assembly   2 terms 
  Biochemical function     protein binding     8 terms  

 

 
    reference    
 
 
PLoS Biol 8: (2010)
PubMed id: 20856905  
 
 
Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
N.Opalka, J.Brown, W.J.Lane, K.A.Twist, R.Landick, F.J.Asturias, S.A.Darst.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
22179134 I.Comas, S.Borrell, A.Roetzer, G.Rose, B.Malla, M.Kato-Maeda, J.Galagan, S.Niemann, and S.Gagneux (2012).
Whole-genome sequencing of rifampicin-resistant Mycobacterium tuberculosis strains identifies compensatory mutations in RNA polymerase genes.
  Nat Genet, 44, 106-110.  
21487437 J.N.Kuehner, E.L.Pearson, and C.Moore (2011).
Unravelling the means to an end: RNA polymerase II transcription termination.
  Nat Rev Mol Cell Biol, 12, 283-294.  
  21029432 E.P.Geiduschek, and G.A.Kassavetis (2010).
Transcription of the T4 late genes.
  Virol J, 7, 288.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.