PDBsum entry 3lti

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protein links
Transferase PDB id
Protein chain
284 a.a. *
Waters ×378
* Residue conservation analysis
PDB id:
Name: Transferase
Title: Crystal structure of the escherichia coli RNA polymerase bet beta2-betai4 domains
Structure: DNA-directed RNA polymerase subunit beta. Chain: a. Fragment: beta2-beta-i-4 domains. Synonym: rnap subunit beta, transcriptase subunit beta, RNA polymerase subunit beta. Engineered: yes
Source: Escherichia coli. Organism_taxid: 83333. Strain: k12. Gene: b3987, gron, jw3950, nitb, rif, ron, rpob, stl, stv, expressed in: escherichia coli. Expression_system_taxid: 562.
1.60Å     R-factor:   0.209     R-free:   0.230
Authors: S.A.Darst,N.Opalka
Key ref: N.Opalka et al. (2010). Complete structural model of Escherichia coli RNA polymerase from a hybrid approach. PLoS Biol, 8, . PubMed id: 20856905
16-Feb-10     Release date:   20-Oct-10    
Go to PROCHECK summary

Protein chain
Pfam   ArchSchema ?
P0A8V2  (RPOB_ECOLI) -  DNA-directed RNA polymerase subunit beta
1342 a.a.
284 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 11 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: E.C.  - DNA-directed Rna polymerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Nucleoside triphosphate
+ RNA(n)
= diphosphate
+ RNA(n+1)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     transcription, DNA-dependent   1 term 
  Biochemical function     DNA binding     3 terms  


PLoS Biol 8: (2010)
PubMed id: 20856905  
Complete structural model of Escherichia coli RNA polymerase from a hybrid approach.
N.Opalka, J.Brown, W.J.Lane, K.A.Twist, R.Landick, F.J.Asturias, S.A.Darst.
No abstract given.


Literature references that cite this PDB file's key reference

  PubMed id Reference
22179134 I.Comas, S.Borrell, A.Roetzer, G.Rose, B.Malla, M.Kato-Maeda, J.Galagan, S.Niemann, and S.Gagneux (2012).
Whole-genome sequencing of rifampicin-resistant Mycobacterium tuberculosis strains identifies compensatory mutations in RNA polymerase genes.
  Nat Genet, 44, 106-110.  
21487437 J.N.Kuehner, E.L.Pearson, and C.Moore (2011).
Unravelling the means to an end: RNA polymerase II transcription termination.
  Nat Rev Mol Cell Biol, 12, 283-294.  
  21029432 E.P.Geiduschek, and G.A.Kassavetis (2010).
Transcription of the T4 late genes.
  Virol J, 7, 288.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.