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PDBsum entry 3lat

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protein ligands metals links
Hydrolase PDB id
3lat
Jmol
Contents
Protein chains
207 a.a. *
Ligands
IMD ×2
BU1 ×3
Metals
_CL
_ZN ×14
Waters ×526
* Residue conservation analysis
PDB id:
3lat
Name: Hydrolase
Title: Crystal structure of staphylococcus peptidoglycan hydrolase amie
Structure: Bifunctional autolysin. Chain: a, b. Fragment: unp residues 303-515. Synonym: peptidoglycan hydrolase amie, atle, n- acetylmuramoyl-l-alanine amidase. Engineered: yes
Source: Staphylococcus epidermidis. Organism_taxid: 1282. Strain: o-47. Gene: atle. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.70Å     R-factor:   0.144     R-free:   0.165
Authors: S.Zoll,T.Stehle
Date:
07-Jan-10     Release date:   23-Mar-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chains
Pfam   ArchSchema ?
O33635  (ATL_STAEP) -  Bifunctional autolysin
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1335 a.a.
207 a.a.
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain

 Enzyme reactions 
   Enzyme class 1: E.C.3.2.1.96  - Mannosyl-glycoprotein endo-beta-N-acetylglucosaminidase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endohydrolysis of the di-N-acetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -[Man(GlcNAc)2]Asn- structure. One N-acetyl-D-glucosamine residue remains attached to the protein; the rest of the oligosaccharide is released intact.
   Enzyme class 2: E.C.3.5.1.28  - N-acetylmuramoyl-L-alanine amidase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain bacterial cell-wall glycopeptides.
Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     peptidoglycan catabolic process   1 term 
  Biochemical function     N-acetylmuramoyl-L-alanine amidase activity     1 term