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PDBsum entry 3ksk

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protein ligands Protein-protein interface(s) links
Hydrolase PDB id
3ksk
Jmol
Contents
Protein chains
313 a.a.
Ligands
SO4 ×5
MPD
TRS ×2
Waters ×469
PDB id:
3ksk
Name: Hydrolase
Title: Crystal structure of single chain pvuii
Structure: Type-2 restriction enzyme pvuii. Chain: a, b. Synonym: r.Pvuii, type ii restriction enzyme pvuii, endonuclease pvuii. Engineered: yes
Source: Proteus vulgaris. Organism_taxid: 585. Gene: pvuii, pvuiir. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.35Å     R-factor:   0.219     R-free:   0.283
Authors: C.Meramveliotaki,A.Hountas,E.Eliopoulos,M.Kokkinidis
Key ref: C.Meramveliotaki et al. Crystal structure of single chain pvuii. To be published, .
Date:
23-Nov-09     Release date:   14-Apr-10    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P23657  (T2P2_PROHU) -  Type-2 restriction enzyme PvuII
Seq:
Struc:
157 a.a.
313 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.1.21.4  - Type Ii site-specific deoxyribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
      Cofactor: Magnesium
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     nucleic acid phosphodiester bond hydrolysis   3 terms 
  Biochemical function     hydrolase activity     5 terms