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PDBsum entry 3kne

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protein ligands metals links
Lyase PDB id
3kne
Jmol
Contents
Protein chain
257 a.a.
Ligands
BE7
DAW
Metals
_ZN
Waters ×219
PDB id:
3kne
Name: Lyase
Title: Carbonic anhydrase ii h64c mutant in complex with an in situ triazole
Structure: Carbonic anhydrase 2. Chain: a. Synonym: carbonic anhydrase ii, ca-ii, carbonate dehydratas carbonic anhydrasE C, cac. Engineered: yes. Mutation: yes
Source: Homo sapiens. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.35Å     R-factor:   0.129     R-free:   0.183
Authors: J.Schulze-Wischeler,A.Heine,G.Klebe
Key ref: J.S.Wischeler et al. (2011). Stereo- and regioselective azide/alkyne cycloadditions in carbonic anhydrase II via tethering, monitored by crystallography and mass spectrometry. Chemistry, 17, 5842-5851. PubMed id: 21506176
Date:
12-Nov-09     Release date:   18-May-11    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P00918  (CAH2_HUMAN) -  Carbonic anhydrase 2
Seq:
Struc:
260 a.a.
257 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.4.2.1.1  - Carbonate dehydratase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: H2CO3 = CO2 + H2O
H(2)CO(3)
= CO(2)
+ H(2)O
      Cofactor: Zn(2+)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular space   11 terms 
  Biological process     angiotensin-mediated signaling pathway   21 terms 
  Biochemical function     protein binding     5 terms  

 

 
    Added reference    
 
 
Chemistry 17:5842-5851 (2011)
PubMed id: 21506176  
 
 
Stereo- and regioselective azide/alkyne cycloadditions in carbonic anhydrase II via tethering, monitored by crystallography and mass spectrometry.
J.S.Wischeler, D.Sun, N.U.Sandner, U.Linne, A.Heine, U.Koert, G.Klebe.
 
  ABSTRACT  
 
No abstract given.