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PDBsum entry 3klk

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protein ligands metals links
Transferase PDB id
3klk
Jmol
Contents
Protein chain
1006 a.a. *
Ligands
GOL ×8
Metals
_CA
Waters ×1055
* Residue conservation analysis
PDB id:
3klk
Name: Transferase
Title: Crystal structure of lactobacillus reuteri n-terminally trun glucansucrase gtf180 in triclinic apo- form
Structure: Glucansucrase. Chain: a. Fragment: n-terminally truncated gtf180, unp residues 742-1 engineered: yes
Source: Lactobacillus reuteri. Organism_taxid: 1598. Strain: 180. Gene: gtf180. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.65Å     R-factor:   0.167     R-free:   0.195
Authors: A.Vujicic-Zagar,T.Pijning,S.Kralj,W.Eeuwema,L.Dijkhuizen,B.W
Key ref: A.Vujicic-Zagar et al. (2010). Crystal structure of a 117 kDa glucansucrase fragment provides insight into evolution and product specificity of GH70 enzymes. Proc Natl Acad Sci U S A, 107, 21406-21411. PubMed id: 21118988
Date:
08-Nov-09     Release date:   03-Nov-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q5SBN3  (Q5SBN3_LACRE) -  Glucansucrase
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
 
Seq:
Struc:
1772 a.a.
1006 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.2.4.1.5  - Dextransucrase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Sucrose + ((1->6)-alpha-D-glucosyl)(n) = D-fructose + ((1->6)-alpha-D- glucosyl)(n+1)
Sucrose
+ ((1->6)-alpha-D-glucosyl)(n)
=
D-fructose
Bound ligand (Het Group name = GOL)
matches with 50.00% similarity
+ ((1->6)-alpha-D- glucosyl)(n+1)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     carbohydrate metabolic process   2 terms 
  Biochemical function     glucosyltransferase activity     1 term  

 

 
    Added reference    
 
 
Proc Natl Acad Sci U S A 107:21406-21411 (2010)
PubMed id: 21118988  
 
 
Crystal structure of a 117 kDa glucansucrase fragment provides insight into evolution and product specificity of GH70 enzymes.
A.Vujicic-Zagar, T.Pijning, S.Kralj, C.A.López, W.Eeuwema, L.Dijkhuizen, B.W.Dijkstra.
 
  ABSTRACT  
 
No abstract given.

 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21354427 K.Ito, S.Ito, T.Shimamura, S.Weyand, Y.Kawarasaki, T.Misaka, K.Abe, T.Kobayashi, A.D.Cameron, and S.Iwata (2011).
Crystal structure of glucansucrase from the dental caries pathogen Streptococcus mutans.
  J Mol Biol, 408, 177-186.
PDB codes: 3aib 3aic 3aie
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB codes are shown on the right.