PDBsum entry 3j3e

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Ribosome PDB id
PDB id:
Name: Ribosome
Title: Structure of the d. Melanogaster 60s rrna
Structure: 28s ribosomal RNA. Chain: 5. 2s ribosomal RNA. Chain: 9. 5s ribosomal RNA. Chain: 7. 5.8s ribosomal RNA. Chain: 8
Source: Drosophila melanogaster. Fruit fly. Organism_taxid: 7227. Organism_taxid: 7227
Authors: A.M.Anger,J.-P.Armache,O.Berninghausen,M.Habeck,M.Subklewe, D.N.Wilson,R.Beckmann
Key ref: A.M.Anger et al. (2013). Structures of the human and Drosophila 80S ribosome. Nature, 497, 80-85. PubMed id: 23636399 DOI: 10.1038/nature12104
27-Feb-13     Release date:   01-May-13    


DOI no: 10.1038/nature12104 Nature 497:80-85 (2013)
PubMed id: 23636399  
Structures of the human and Drosophila 80S ribosome.
A.M.Anger, J.P.Armache, O.Berninghausen, M.Habeck, M.Subklewe, D.N.Wilson, R.Beckmann.
Protein synthesis in all cells is carried out by macromolecular machines called ribosomes. Although the structures of prokaryotic, yeast and protist ribosomes have been determined, the more complex molecular architecture of metazoan 80S ribosomes has so far remained elusive. Here we present structures of Drosophila melanogaster and Homo sapiens 80S ribosomes in complex with the translation factor eEF2, E-site transfer RNA and Stm1-like proteins, based on high-resolution cryo-electron-microscopy density maps. These structures not only illustrate the co-evolution of metazoan-specific ribosomal RNA with ribosomal proteins but also reveal the presence of two additional structural layers in metazoan ribosomes, a well-ordered inner layer covered by a flexible RNA outer layer. The human and Drosophila ribosome structures will provide the basis for more detailed structural, biochemical and genetic experiments.