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Structural genomics, unknown function
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PDB id
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3iwt
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Contents |
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* Residue conservation analysis
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PDB id:
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Structural genomics, unknown function
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Title:
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Structure of hypothetical molybdenum cofactor biosynthesis p from sulfolobus tokodaii
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Structure:
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178aa long hypothetical molybdenum cofactor biosy protein b. Chain: a, b, c. Engineered: yes
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Source:
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Sulfolobus tokodaii. Organism_taxid: 111955. Strain: 7, dsm 16993, jcm 10545, nbrc 100140. Gene: st2315. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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1.90Å
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R-factor:
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0.169
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R-free:
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0.187
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Authors:
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S.V.Antonyuk,M.J.Ellis,R.W.Strange,S.S.Hasnain,Y.Bessho,S.Ku S.Yokoyama,Riken Structural Genomics/proteomics Initiative
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Key ref:
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S.V.Antonyuk
et al.
(2009).
Structure of hypothetical Mo-cofactor biosynthesis protein B (ST2315) from Sulfolobus tokodaii.
Acta Crystallogr Sect F Struct Biol Cryst Commun,
65,
1200-1203.
PubMed id:
DOI:
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Date:
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03-Sep-09
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Release date:
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29-Sep-09
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Supersedes:
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PROCHECK
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Headers
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References
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Q96Y52
(Q96Y52_SULTO) -
Molybdopterin biosynthesis protein MoaB
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Seq:
Struc:
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178 a.a.
166 a.a.
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Key: |
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PfamA domain |
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Secondary structure |
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CATH domain |
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Gene Ontology (GO) functional annotation
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Biological process
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Mo-molybdopterin cofactor biosynthetic process
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1 term
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DOI no:
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Acta Crystallogr Sect F Struct Biol Cryst Commun
65:1200-1203
(2009)
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PubMed id:
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Structure of hypothetical Mo-cofactor biosynthesis protein B (ST2315) from Sulfolobus tokodaii.
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S.V.Antonyuk,
R.W.Strange,
M.J.Ellis,
Y.Bessho,
S.Kuramitsu,
A.Shinkai,
S.Yokoyama,
S.S.Hasnain.
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ABSTRACT
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The structure of a probable Mo-cofactor biosynthesis protein B from Sulfolobus
tokodaii, belonging to space group P6(4)22 with unit-cell parameters a = b =
136.68, c = 210.52 A, was solved by molecular replacement to a resolution of 1.9
A and refined to an R factor and R(free) of 16.8% and 18.5%, respectively. The
asymmetric unit contains a trimer, while the biologically significant oligomer
is predicted to be a hexamer by size-exclusion chromatography. The subunit
structure and fold of ST2315 are similar to those of other enzymes that are
known to be involved in the molybdopterin- and molybdenum cofactor-biosynthesis
pathways.
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