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PDBsum entry 3ima

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protein ligands Protein-protein interface(s) links
Hydrolase/hydrolase inhibitor PDB id
3ima
Jmol
Contents
Protein chains
212 a.a. *
84 a.a. *
Ligands
ACT ×3
Waters ×576
* Residue conservation analysis
PDB id:
3ima
Name: Hydrolase/hydrolase inhibitor
Title: Complex strcuture of tarocystatin and papain
Structure: Papain. Chain: a, c. Fragment: papain domain. Synonym: papaya proteinase i, ppi. Cysteine proteinase inhibitor. Chain: b, d. Fragment: n-terminal domain, unp residues 2-92. Synonym: tarocystatin, cecpi. Engineered: yes
Source: Carica papaya. Mamon. Organism_taxid: 3649. Other_details: papain was purchased from sigma co. For co- crystallization with cecpi.. Colocasia esculenta. Cocoyam,dasheen,eddo,elephant's ear,kalo, malanga. Organism_taxid: 4460.
Resolution:
2.03Å     R-factor:   0.182     R-free:   0.233
Authors: M.H.Chu,K.L.Liu,K.W.Yeh,Y.S.Cheng
Key ref: M.H.Chu et al. Complex strcuture of tarocystatin and papain. To be published, .
Date:
10-Aug-09     Release date:   16-Feb-10    
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
P00784  (PAPA1_CARPA) -  Papain
Seq:
Struc:
345 a.a.
212 a.a.*
Protein chains
Pfam   ArchSchema ?
Q8L5J8  (Q8L5J8_COLES) -  Cysteine proteinase inhibitor
Seq:
Struc:
205 a.a.
84 a.a.
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: Chains A, C: E.C.3.4.22.2  - Papain.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Hydrolysis of proteins with broad specificity for peptide bonds, with preference for a residue bearing a large hydrophobic sidechain at the P2 position. Does not accept Val at P1'.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     proteolysis   1 term 
  Biochemical function     cysteine-type peptidase activity     2 terms