 |
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Isomerase
|
|
|
Title:
|
|
Structure of DNA gyrase subunit a n-terminal domain
|
|
Structure:
|
|
DNA gyrase subunit a. Chain: a, b. Fragment: n-terminal domain. Engineered: yes
|
|
Source:
|
|
Mycobacterium tuberculosis. Organism_taxid: 1773. Gene: gyra, mt0006, mtcy10h4.04, rv0006. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
|
|
Resolution:
|
|
|
1.60Å
|
R-factor:
|
0.178
|
R-free:
|
0.201
|
|
|
Authors:
|
|
E.M.Tretter,A.J.Schoeffler,S.R.Weisfield,J.M.Berger,Tb Struc Genomics Consortium (Tbsgc)
|
Key ref:
|
|
E.M.Tretter
et al.
(2010).
Crystal structure of the DNA gyrase GyrA N-terminal domain from Mycobacterium tuberculosis.
Proteins,
78,
492-495.
PubMed id:
DOI:
|
|
|
Date:
|
|
|
07-Aug-09
|
Release date:
|
06-Oct-09
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
Q07702
(GYRA_MYCTU) -
DNA gyrase subunit A
|
|
|
|
Seq:
Struc:
|
|
|
|
838 a.a.
464 a.a.
|
|
|
|
|
|
|
|
|
|
|
|
|
Key: |
 |
PfamA domain |
|
|
 |
Secondary structure |
|
|
|
|
|
|
|
|
|
|
|
|
|
Enzyme class:
|
|
E.C.5.99.1.3
- Dna topoisomerase (ATP-hydrolyzing).
|
|
|
|
|
|
|
Reaction:
|
|
ATP-dependent breakage, passage and rejoining of double-stranded DNA.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Gene Ontology (GO) functional annotation
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Cellular component
|
chromosome
|
1 term
|
|
|
Biological process
|
DNA metabolic process
|
3 terms
|
|
|
Biochemical function
|
DNA binding
|
4 terms
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
DOI no:
|
Proteins
78:492-495
(2010)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Crystal structure of the DNA gyrase GyrA N-terminal domain from Mycobacterium tuberculosis.
|
|
E.M.Tretter,
A.J.Schoeffler,
S.R.Weisfield,
J.M.Berger.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
|
|
| |
Selected figure(s)
|
|
|
|
| |
|
|
|
|
Figure 1.
Figure 1. MtGyr59 structure. (A) Representative electron
density from a refined 2F[o]-F[c] map contoured at 2  .
(B) Representation (Pymol[18]) of the MtGyr59 dimer. The active
site Tyrosine (Tyr129) is highlighted pink. (C) Structural
alignments of the MtGyr59 (yellow) and EcGyrA59 (fuchsia) helix
 4
region. Gly88 and Asp82 are shown as sticks overlaid with
transparent space filling spheres to highlight the clashes that
would occur if MtGyr59 region adopted the same structure as seen
in EcGyrA59.[9] Asp89 of MtGyrA59 is also shown, together with
moxifloxacin superposed from the S. pneumoniae topo IV
co-crystal structure,[15] to highlight how the MtGyr59 loop
would clash with bound drug. (D) Overlay of the DNA bending loop
region between MtGyr59 (yellow) and EcGyrA59 (fuchsia).
|
|
|
|
|
|
| |
The above figure is
reprinted
by permission from John Wiley & Sons, Inc.:
Proteins
(2010,
78,
492-495)
copyright 2010.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Literature references that cite this PDB file's key reference
|
|
|
| |
PubMed id
|
|
Reference
|
|
|
|
|
|
A.J.Schoeffler,
A.P.May,
and
J.M.Berger
(2010).
A domain insertion in Escherichia coli GyrB adopts a novel fold that plays a critical role in gyrase function.
|
| |
Nucleic Acids Res, 38,
7830-7844.
|
|
|
PDB code:
|
|
|
|
|
|
|
|
B.D.Bax,
P.F.Chan,
D.S.Eggleston,
A.Fosberry,
D.R.Gentry,
F.Gorrec,
I.Giordano,
M.M.Hann,
A.Hennessy,
M.Hibbs,
J.Huang,
E.Jones,
J.Jones,
K.K.Brown,
C.J.Lewis,
E.W.May,
M.R.Saunders,
O.Singh,
C.E.Spitzfaden,
C.Shen,
A.Shillings,
A.J.Theobald,
A.Wohlkonig,
N.D.Pearson,
and
M.N.Gwynn
(2010).
Type IIA topoisomerase inhibition by a new class of antibacterial agents.
|
| |
Nature, 466,
935-940.
|
|
|
PDB codes:
|
|
|
|
|
|
|
|
J.Piton,
S.Petrella,
M.Delarue,
G.André-Leroux,
V.Jarlier,
A.Aubry,
and
C.Mayer
(2010).
Structural insights into the quinolone resistance mechanism of Mycobacterium tuberculosis DNA gyrase.
|
| |
PLoS One, 5,
e12245.
|
|
|
PDB codes:
|
|
|
|
|
|
|
The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
|
|
Links
