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PDBsum entry 3ie0

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protein ligands metals Protein-protein interface(s) links
Hydrolase PDB id
3ie0
Jmol
Contents
Protein chains
431 a.a. *
Ligands
SO4 ×67
FLC ×3
Metals
_ZN ×8
Waters ×80
* Residue conservation analysis
PDB id:
3ie0
Name: Hydrolase
Title: Crystal structure of s378y mutant ttha0252 from thermus ther hb8
Structure: Ribonuclease ttha0252. Chain: a, b, c, d. Engineered: yes. Mutation: yes
Source: Thermus thermophilus. Organism_taxid: 300852. Strain: hb8. Gene: ttha0252. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Resolution:
2.73Å     R-factor:   0.218     R-free:   0.272
Authors: H.Ishikawa,N.Nakagawa,S.Kuramitsu,S.Yokoyama,R.Masui,Riken S Genomics/proteomics Initiative (Rsgi)
Key ref: H.Ishikawa et al. Crystal structure of s378y mutant ttha0252 from thermus thermophilus hb8. To be published, .
Date:
22-Jul-09     Release date:   04-Aug-09    
Supersedes: 2zdd
PROCHECK
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 Headers
 References

Protein chains
Pfam   ArchSchema ?
Q5SLP1  (RNSE_THET8) -  Ribonuclease TTHA0252
Seq:
Struc:
431 a.a.
431 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   1 term 
  Biological process     nucleic acid phosphodiester bond hydrolysis   2 terms 
  Biochemical function     hydrolase activity     5 terms