spacer
spacer

PDBsum entry 3i7y

Go to PDB code: 
protein metals links
Hydrolase PDB id
3i7y
Jmol
Contents
Protein chain
124 a.a. *
Metals
_CL ×3
Waters ×76
* Residue conservation analysis
PDB id:
3i7y
Name: Hydrolase
Title: High pressure structure of i106a variant of rnase a (0.48 gpa)
Structure: Ribonuclease pancreatic. Chain: a. Synonym: rnase 1, rnase a. Engineered: yes. Mutation: yes
Source: Bos taurus. Bovine. Organism_taxid: 9913. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.
Resolution:
2.40Å     R-factor:   0.200     R-free:   0.241
Authors: K.Lewinski,K.Kurpiewska,K.Dziubek,A.Katrusiak,J.Font,M.Ribo, M.Vilanova
Key ref: K.Lewinski et al. X-Ray diffraction studies on rnase a crystal structure at high pressure. To be published, .
Date:
09-Jul-09     Release date:   04-Aug-09    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
P61823  (RNAS1_BOVIN) -  Ribonuclease pancreatic
Seq:
Struc:
150 a.a.
124 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.1.27.5  - Pancreatic ribonuclease.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotides ending in C-P or U-P with 2',3'-cyclic phosphate intermediates.
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   1 term 
  Biological process     metabolic process   3 terms 
  Biochemical function     nucleic acid binding     7 terms