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Hydrolase/hydrolase regulator PDB id
3hna
Jmol
Contents
Protein chains
280 a.a. *
260 a.a. *
11 a.a. *
Ligands
LYS-GLN-THR-ALA-
ARG-MLZ-SER-THR
SAH ×2
Metals
_ZN ×8
Waters ×888
* Residue conservation analysis
PDB id:
3hna
Name: Hydrolase/hydrolase regulator
Title: Crystal structure of catalytic domain of human euchromatic histone methyltransferase 1 in complex with sah and mono- methylated h3k9 peptide
Structure: Histone-lysine n-methyltransferase, h3 lysine-9 specific 5. Chain: a, b. Synonym: histone h3-k9 methyltransferase 5, h3-k9-hmtase 5, euchromatic histone-lysine n-methyltransferase 1, eu- hmtase1, g9a-like protein 1, glp1, lysine n- methyltransferase 1d. Engineered: yes. Mono-methylated h3k9 peptide.
Source: Homo sapiens. Human. Organism_taxid: 9606. Strain: bl21(de3). Gene: ehmt1, euhmtase1, kiaa1876, kmt1d. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes
Resolution:
1.50Å     R-factor:   0.167     R-free:   0.193
Authors: J.Min,H.Wu,P.Loppnau,J.Wleigelt,M.Sundstrom,C.H.Arrowsmith, A.M.Edwards,A.Bochkarev,A.N.Plotnikov,Structural Genomics Consortium (Sgc)
Date:
30-May-09     Release date:   09-Jun-09    
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9H9B1  (EHMT1_HUMAN) -  Histone-lysine N-methyltransferase EHMT1
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1298 a.a.
280 a.a.*
Protein chain
Pfam   ArchSchema ?
Q9H9B1  (EHMT1_HUMAN) -  Histone-lysine N-methyltransferase EHMT1
Seq:
Struc: 		 
Seq:
Struc: 		 
Seq:
Struc: 		1298 a.a.
260 a.a.
Protein chain
No UniProt id for this chain
Key:    PfamA domain  PfamB domain  Secondary structure
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chains A, B: E.C.2.1.1.43  - Histone-lysine N-methyltransferase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: S-adenosyl-L-methionine + L-lysine-[histone] = S-adenosyl-L-homocysteine + N6-methyl-L-lysine-[histone]
S-adenosyl-L-methionine
 + L-lysine-[histone]
 =
S-adenosyl-L-homocysteine
Bound ligand (Het Group name = SAH)
corresponds exactly 		+ N(6)-methyl-L-lysine-[histone]
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     nucleus   1 term 
  Biological process     chromatin modification   1 term 
  Biochemical function     zinc ion binding     2 terms