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PDBsum entry 3h5e

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protein Protein-protein interface(s) links
Lyase PDB id
3h5e
Jmol
Contents
Protein chains
154 a.a. *
Waters ×135
* Residue conservation analysis
PDB id:
3h5e
Name: Lyase
Title: Leud_1-156 small subunit of isopropylmalate isomerase (rv298 mycobacterium tuberculosis
Structure: 3-isopropylmalate dehydratase small subunit. Chain: a, b. Fragment: leud, residues 1-156. Synonym: isopropylmalate isomerase small subunit, alpha-ipm isomerase, ipmi. Engineered: yes
Source: Mycobacterium tuberculosis. Organism_taxid: 1773. Strain: h37rv. Gene: rv2987c. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
2.00Å     R-factor:   0.235     R-free:   0.269
Authors: K.Manikandan,A.Geerlof,M.S.Weiss
Key ref: K.Manikandan et al. (2011). Structural studies on the enzyme complex isopropylmalate isomerase (LeuCD) from Mycobacterium tuberculosis. Proteins, 79, 35-49. PubMed id: 20938981
Date:
22-Apr-09     Release date:   07-Apr-10    
PROCHECK
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 Headers
 References

Protein chains
Pfam  
P9WK94  (LEUD_MYCTO) -  3-isopropylmalate dehydratase small subunit
Seq:
Struc:
198 a.a.
154 a.a.*
Key:    Secondary structure  CATH domain
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.4.2.1.33  - 3-isopropylmalate dehydratase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
Leucine Biosynthesis
      Reaction: (2R,3S)-3-isopropylmalate = (2S)-2-isopropylmalate
(2R,3S)-3-isopropylmalate
= (2S)-2-isopropylmalate
      Cofactor: Iron-sulfur
Iron-sulfur
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     3-isopropylmalate dehydratase complex   1 term 
  Biological process     metabolic process   2 terms 
  Biochemical function     3-isopropylmalate dehydratase activity     1 term  

 

 
    reference    
 
 
Proteins 79:35-49 (2011)
PubMed id: 20938981  
 
 
Structural studies on the enzyme complex isopropylmalate isomerase (LeuCD) from Mycobacterium tuberculosis.
K.Manikandan, A.Geerlof, A.V.Zozulya, D.I.Svergun, M.S.Weiss.
 
  ABSTRACT  
 
No abstract given.