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PDBsum entry 3h4x

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protein ligands metals links
Hydrolase PDB id
3h4x
Jmol
Contents
Protein chain
315 a.a. *
Ligands
EOH ×20
ACT ×3
GOL ×3
ACE ×5
Metals
_CL
Waters ×386
* Residue conservation analysis
PDB id:
3h4x
Name: Hydrolase
Title: Structure of a ca+2 dependent phosphatidylinositol-specific phospholipasE C (pi-plc) enzyme from streptomyces antibioti
Structure: Phosphatidylinositol-specific phospholipase c1. Chain: a. Engineered: yes
Source: Streptomyces antibioticus. Organism_taxid: 1890. Gene: plc1, plc1 ab439135.1:1234..2268. Expressed in: escherichia coli. Expression_system_taxid: 562.
Resolution:
1.23Å     R-factor:   0.174     R-free:   0.190
Authors: M.R.Jackson,T.L.Selby
Key ref: M.R.Jackson and t.l.selby Crystal structure of a ca2+-Dependent pi-Plc. To be published, .
Date:
21-Apr-09     Release date:   12-May-10    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam  
B3A043  (B3A043_STRAT) -  Phosphatidylinositol-specific phospholipase C1
Seq:
Struc:
344 a.a.
315 a.a.*
Key:    Secondary structure
* PDB and UniProt seqs differ at 1 residue position (black cross)

 Enzyme reactions 
   Enzyme class: E.C.3.1.4.11  - Phosphoinositide phospholipase C.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]

      Pathway:
myo-Inositol Phosphate Metabolism
      Reaction: 1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate + H2O = 1D-myo-inositol 1,4,5-trisphosphate + diacylglycerol
1-phosphatidyl-1D-myo-inositol 4,5-bisphosphate
+ H(2)O
= 1D-myo-inositol 1,4,5-trisphosphate
+
diacylglycerol
Bound ligand (Het Group name = GOL)
matches with 50.00% similarity
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Biological process     lipid metabolic process   1 term 
  Biochemical function     hydrolase activity     3 terms