PDBsum entry: 3gvo

RNA binding protein

PDB id: 3gvo

Contents

Protein chain

342 a.a. *

Ligands

GOL ×4

DTD

Waters ×423

* Residue conservation analysis

PDB id:

3gvo

Name:

RNA binding protein

Title:

Structure and RNA binding of the mouse pumilio-2 puf domain

Structure:

Pumilio homolog 2. Chain: a. Fragment: puf domain, unp residues 706-1056. Synonym: pumilio 2. Engineered: yes

Source:

Mus musculus. Mouse. Organism_taxid: 10090. Gene: pum2. Expressed in: escherichia coli. Expression_system_taxid: 562.

Resolution:

1.60Å R-factor: 0.168 R-free: 0.198

Authors:

H.T.Jenkins,T.A.Edwards

Key ref:

H.T.Jenkins et al. (2009). Structure and RNA binding of the mouse Pumilio-2 Puf domain. J Struct Biol, 167, 271-276. PubMed id: 19540345 DOI: 10.1016/j.jsb.2009.06.007

Date:

31-Mar-09 Release date: 30-Jun-09

Protein chain

Q80U58  (PUM2_MOUSE) -  Pumilio homolog 2

Seq: 1066 a.a.

Struc: 342 a.a.

 Gene Ontology (GO) functional annotation 

• Biochemical function: binding (2 terms)

DOI no: 10.1016/j.jsb.2009.06.007

J Struct Biol 167:271-276 (2009)

PubMed id: 19540345

Structure and RNA binding of the mouse Pumilio-2 Puf domain.

H.T.Jenkins, R.Baker-Wilding, T.A.Edwards.

ABSTRACT

Puf proteins control translation through the interaction of a C-terminal Puf domain with specific sequences present in the 3' untranslated region of messenger RNAs. In Drosophila, binding of the protein Pumilio to mRNA leads to translational repression which is required for anterior/posterior patterning during embryogenesis. The vertebrate Pumilio homologue 2 (Pum2) has been implicated in controlling germ cell development through interactions with the RNA binding proteins deleted in azoospermia (DAZ), DAZ-like (DAZL) and BOULE. We present the 1.6A resolution X-ray crystal structure of the Puf domain from murine Pum2 and demonstrate that this domain is capable of binding with nanomolar affinity to RNA sequences from the hunchback Nanos response element (NRE) and a previously identified Pum2 binding element (PBE).