PDBsum entry: 3gkl

Hydrolase

PDB id: 3gkl

Contents

Protein chains

120 a.a. *

82 a.a. *

Metals

_ZN ×2

Waters ×10

* Residue conservation analysis

PDB id:

3gkl

Name:

Hydrolase

Title:

Following evolutionary paths to high affinity and selectivity protein-protein interactions using colicin7 and immunity proteins

Structure:

Colicin-e9 immunity protein. Chain: a, b. Fragment: unp residues 446-576. Engineered: yes. Mutation: yes. Colicin-e7. Chain: c, d. Synonym: imme9, microcin-e9 immunity protein. Engineered: yes.

Source:

Escherichia coli. Organism_taxid: 562. Gene: cole7, cea. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: imm, ceie9.

Resolution:

2.20Å R-factor: 0.241 R-free: 0.277

Authors:

O.Dym,D.S.Tawfik,Israel Structural Proteomics Center (Ispc)

Date:

11-Mar-09 Release date: 08-Sep-09

Protein chains

Q47112  (CEA7_ECOLX) -  Colicin-E7

Seq: 576 a.a.

Struc: 120 a.a.*

Protein chains

P13479  (IMM9_ECOLX) -  Colicin-E9 immunity protein

Seq: 86 a.a.

Struc: 82 a.a.*

* PDB and UniProt seqs differ at 9 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 

• Biological process: cytolysis (4 terms)
• Biochemical function: protein binding (4 terms)