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PDBsum entry 3gfk

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protein Protein-protein interface(s) links
Transcription/transferase PDB id
3gfk
Jmol
Contents
Protein chains
118 a.a. *
76 a.a. *
Waters ×257
* Residue conservation analysis
PDB id:
3gfk
Name: Transcription/transferase
Title: Crystal structure of bacillus subtilis spx/RNA polymerase alpha subunit c-terminal domain complex
Structure: Regulatory protein spx. Chain: a. Engineered: yes. DNA-directed RNA polymerase subunit alpha. Chain: b. Fragment: alpha c-terminal domain (alpha-ctd). Synonym: rnap subunit alpha, transcriptase subunit alpha, RNA polymerase subunit alpha. Engineered: yes
Source: Bacillus subtilis. Organism_taxid: 1423. Gene: spxa, bsu11500. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: rpoa, bsu01430. Expression_system_taxid: 562
Resolution:
2.30Å     R-factor:   0.229     R-free:   0.282
Authors: V.Lamour,L.F.Westblade,E.A.Campbell,S.A.Darst
Key ref: V.Lamour et al. (2009). Crystal structure of the in vivo-assembled Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex. J Struct Biol, 168, 352-356. PubMed id: 19580872
Date:
27-Feb-09     Release date:   10-Mar-09    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
O31602  (SPX_BACSU) -  Regulatory protein Spx
Seq:
Struc:
131 a.a.
118 a.a.
Protein chain
Pfam   ArchSchema ?
P20429  (RPOA_BACSU) -  DNA-directed RNA polymerase subunit alpha
Seq:
Struc:
314 a.a.
76 a.a.*
Key:    PfamA domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 4 residue positions (black crosses)

 Enzyme reactions 
   Enzyme class: Chain B: E.C.2.7.7.6  - DNA-directed Rna polymerase.
[IntEnz]   [ExPASy]   [KEGG]   [BRENDA]
      Reaction: Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Nucleoside triphosphate
+ RNA(n)
= diphosphate
+ RNA(n+1)
Molecule diagrams generated from .mol files obtained from the KEGG ftp site
 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     cytoplasm   1 term 
  Biological process     transcription, DNA-dependent   3 terms 
  Biochemical function     protein binding     3 terms  

 

 
    reference    
 
 
J Struct Biol 168:352-356 (2009)
PubMed id: 19580872  
 
 
Crystal structure of the in vivo-assembled Bacillus subtilis Spx/RNA polymerase alpha subunit C-terminal domain complex.
V.Lamour, L.F.Westblade, E.A.Campbell, S.A.Darst.
 
  ABSTRACT  
 
The Bacillus subtilis Spx protein is a global transcription factor that interacts with the C-terminal domain of the RNA polymerase alpha subunit (alphaCTD) and regulates transcription of genes involved in thiol-oxidative stress, sporulation, competence, and organosulfur metabolism. Here we determined the X-ray crystal structure of the Spx/alphaCTD complex from an entirely new crystal form than previously reported [Newberry, K.J., Nakano, S., Zuber, P., Brennan, R.G., 2005. Crystal structure of the Bacillus subtilis anti-alpha, global transcriptional regulator, Spx, in complex with the alpha C-terminal domain of RNA polymerase. Proc. Natl. Acad. Sci. USA 102, 15839-15844]. Comparison of the previously reported sulfate-bound complex and our sulfate-free complex reveals subtle conformational changes that may be important for the role of Spx in regulating organosulfur metabolism.
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
20626317 H.Antelmann, and J.D.Helmann (2011).
Thiol-based redox switches and gene regulation.
  Antioxid Redox Signal, 14, 1049-1063.  
20084284 M.M.Nakano, A.Lin, C.S.Zuber, K.J.Newberry, R.G.Brennan, and P.Zuber (2010).
Promoter recognition by a complex of Spx and the C-terminal domain of the RNA polymerase alpha subunit.
  PLoS One, 5, e8664.
PDB code: 3ihq
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.