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protein ligands metals Protein-protein interface(s) links
Structural protein PDB id
3edl
Jmol
Contents
Protein chains
412 a.a. *
426 a.a. *
301 a.a. *
Ligands
GTP ×2
GDP ×2
TA1
ANP
CN2
Metals
_MG ×3
_ZN
Waters ×107
* Residue conservation analysis
PDB id:
3edl
Name: Structural protein
Title: Kinesin13-microtubule ring complex
Structure: Alpha-tubulin. Chain: a. Beta tubulin. Chain: b, g. Kinesin13 motor domain. Chain: d. Engineered: yes. Alpha-tubulin. Chain: f
Source: Bos taurus. Bovine. Tissue: brain. Drosophila melanogaster. Organism_taxid: 7227. Gene: dmklp10a. Expressed in: escherichia coli. Expression_system_taxid: 562. Tissue: brain
Authors: D.Tan,W.J.Rice,H.Sosa
Key ref:
D.Tan et al. (2008). Structure of the kinesin13-microtubule ring complex. Structure, 16, 1732-1739. PubMed id: 19000825 DOI: 10.1016/j.str.2008.08.017
Date:
03-Sep-08     Release date:   20-Jan-09    
PROCHECK
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 Headers
 References

 

 
DOI no: 10.1016/j.str.2008.08.017 Structure 16:1732-1739 (2008)
PubMed id: 19000825  
 
 
Structure of the kinesin13-microtubule ring complex.
D.Tan, W.J.Rice, H.Sosa.
 
  ABSTRACT  
 
To investigate the mechanism of kinesin13-induced microtubule depolymerization, we have calculated a three-dimensional (3D) map of the kinesin13-microtubule ring complex, using cryo-electron microscopy (cryo-EM) and image analysis. An atomic model of the complex was produced by docking the crystal structures of tubulin and a kinesin13 motor domain (MD) into the 3D map. The model reveals a snapshot of the depolymerization mechanism by providing a 3D view of the complex formed between the kinesin13 MD and a curved tubulin protofilament (pf). It suggests that contacts mediated by kinesin13 class-specific residues in the putative microtubule-binding site stabilize intra-dimer tubulin curvature. In addition, a tubulin-binding site on the kinesin13 MD was identified. Mutations at this class-conserved site selectively disrupt the formation of microtubule-associated ring complexes.
 
  Selected figure(s)  
 
Figure 1.
Figure 1. Human Kinesin13s KIF2A and KIF2C MD Form Rings Around Microtubules in the Presence of AMP-PNP
(A) Homo sapiens KIF2A
(B) H. sapiens KIF2C
(C) D. melanogaster KLP10A. Scale bar is 50 nm. 		Figure 4.
Figure 4. Comparison of Kinesin-Tubulin Complexes
For comparison, the kinesin13-curved tubulin complex and a recent kinesin1-microtubule complex model (PDB identification code 2p4n) (Sindelar and Downing, 2007) were superimposed by aligning conserved residues of the kinesin MDs.
(A) Yellow: straight tubulin heterodimer from the kinesin1-microtubule complex model. Red: kinesin1 MD. Blue: kinesin13 MD.
(B) Yellow: curved tubulin from the kinesin13-curved tubulin complex. Red: kinesin1 MD. Blue: kinesin13 MD. Regions α-helix-4 and Loop-2 containing the kinesin13 class specific sequences KEC and KVD are indicated.
 
  The above figures are reprinted by permission from Cell Press: Structure (2008, 16, 1732-1739) copyright 2008.  
  Figures were selected by an automated process.  

Literature references that cite this PDB file's key reference

  PubMed id Reference
20818331 C.Peters, K.Brejc, L.Belmont, A.J.Bodey, Y.Lee, M.Yu, J.Guo, R.Sakowicz, J.Hartman, and C.A.Moores (2010).
Insight into the molecular mechanism of the multitasking kinesin-8 motor.
  EMBO J, 29, 3437-3447.
PDB code: 3lre
20221784 G.Civelekoglu-Scholey, and J.M.Scholey (2010).
Mitotic force generators and chromosome segregation.
  Cell Mol Life Sci, 67, 2231-2250.  
19332892 A.M.Mulder, A.Glavis-Bloom, C.A.Moores, M.Wagenbach, B.Carragher, L.Wordeman, and R.A.Milligan (2009).
A new model for binding of kinesin 13 to curved microtubule protofilaments.
  J Cell Biol, 185, 51-57.  
19530174 A.Marx, A.Hoenger, and E.Mandelkow (2009).
Structures of kinesin motor proteins.
  Cell Motil Cytoskeleton, 66, 958-966.  
19779463 M.Osawa, D.E.Anderson, and H.P.Erickson (2009).
Curved FtsZ protofilaments generate bending forces on liposome membranes.
  EMBO J, 28, 3476-3484.  
19793918 U.Rath, G.C.Rogers, D.Tan, M.A.Gomez-Ferreria, D.W.Buster, H.J.Sosa, and D.J.Sharp (2009).
The Drosophila kinesin-13, KLP59D, impacts Pacman- and Flux-based chromosome movement.
  Mol Biol Cell, 20, 4696-4705.  
19687256 V.Mennella, D.Y.Tan, D.W.Buster, A.B.Asenjo, U.Rath, A.Ma, H.J.Sosa, and D.J.Sharp (2009).
Motor domain phosphorylation and regulation of the Drosophila kinesin 13, KLP10A.
  J Cell Biol, 186, 481-490.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.