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Protein binding
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PDB id
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3e4b
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Contents |
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* Residue conservation analysis
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PDB id:
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Protein binding
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Title:
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Crystal structure of algk from pseudomonas fluorescens wcs37
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Structure:
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Algk. Chain: a, b, c, d. Engineered: yes. Mutation: yes
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Source:
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Pseudomonas fluorescens. Organism_taxid: 294. Strain: wcs374r. Gene: algk. Expressed in: escherichia coli. Expression_system_taxid: 562.
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Resolution:
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2.50Å
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R-factor:
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0.220
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R-free:
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0.280
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Authors:
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C.-L.Keiski,M.Harwich,S.Jain,A.M.Neculai,P.Yip,H.Robinson, J.C.Whitney,L.L.Burrows,D.E.Ohman,P.L.Howell
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Key ref:
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C.L.Keiski
et al.
(2010).
AlgK is a TPR-containing protein and the periplasmic component of a novel exopolysaccharide secretin.
Structure,
18,
265-273.
PubMed id:
DOI:
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Date:
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11-Aug-08
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Release date:
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25-Aug-09
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PROCHECK
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Headers
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References
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DOI no:
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Structure
18:265-273
(2010)
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PubMed id:
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AlgK is a TPR-containing protein and the periplasmic component of a novel exopolysaccharide secretin.
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C.L.Keiski,
M.Harwich,
S.Jain,
A.M.Neculai,
P.Yip,
H.Robinson,
J.C.Whitney,
L.Riley,
L.L.Burrows,
D.E.Ohman,
P.L.Howell.
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ABSTRACT
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The opportunistic pathogen Pseudomonas aeruginosa causes chronic biofilm
infections in cystic fibrosis patients. During colonization of the lung, P.
aeruginosa converts to a mucoid phenotype characterized by overproduction of the
exopolysaccharide alginate. Here we show that AlgK, a protein essential for
production of high molecular weight alginate, is an outer membrane lipoprotein
that contributes to the correct localization of the porin AlgE. Our 2.5 A
structure shows AlgK is composed of 9.5 tetratricopeptide-like repeats, and
three putative sites of protein-protein interaction have been identified.
Bioinformatics analysis suggests that BcsA, PgaA, and PelB, involved in the
production and export of cellulose, poly-beta-1,6-N-Acetyl-D-glucosamine, and
Pel exopolysaccharide, respectively, share the same topology as AlgK/E.
Together, our data suggest that AlgK plays a role in the assembly of the
alginate biosynthetic complex and represents the periplasmic component of a new
type of outer membrane secretin that differs from canonical bacterial capsular
polysaccharide secretion systems.
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Literature references that cite this PDB file's key reference
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PubMed id
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Reference
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C.Whitfield,
and
I.L.Mainprize
(2010).
TPR motifs: hallmarks of a new polysaccharide export scaffold.
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Structure, 18,
151-153.
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J.T.Weadge,
P.P.Yip,
H.Robinson,
K.Arnett,
P.A.Tipton,
and
P.L.Howell
(2010).
Expression, purification, crystallization and preliminary X-ray analysis of Pseudomonas aeruginosa AlgX.
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Acta Crystallogr Sect F Struct Biol Cryst Commun, 66,
588-591.
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The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
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Links
