 |
PDBsum entry 3e2h
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Immune system
|
PDB id
|
|
|
|
3e2h
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
Contents |
 |
|
|
|
|
|
|
|
|
|
|
|
175 a.a.
|
|
|
|
|
|
|
|
|
|
|
109 a.a.
|
|
|
|
|
|
|
|
|
|
|
110 a.a.
|
|
|
|
|
|
|
|
|
* Residue conservation analysis
|
|
|
|
|
|
PDB id:
|
|
|
|
| Name: |
|
Immune system
|
|
|
Title:
|
|
Structure of the m67 high-affinity mutant of the 2c tcr in complex with ld/ql9
|
|
Structure:
|
|
H-2 class i histocompatibility antigen, l-d alpha chain. Chain: a. Engineered: yes. Mutation: yes. Ql9 peptide. Chain: q. Engineered: yes. T-cell receptor alpha chain v region phds58. Chain: b.
|
|
Source:
|
|
Mus musculus. Mouse. Organism_taxid: 10090. Gene: h2-l. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: this sequence occurs naturally in mouse.
|
|
Resolution:
|
|
|
3.80Å
|
R-factor:
|
0.223
|
R-free:
|
0.276
|
|
|
Authors:
|
|
L.A.Colf,K.C.Garcia
|
|
Key ref:
|
|
L.L.Jones
et al.
(2008).
Distinct CDR3 conformations in TCRs determine the level of cross-reactivity for diverse antigens, but not the docking orientation.
J Immunol,
181,
6255-6264.
PubMed id:
|
|
|
Date:
|
|
|
05-Aug-08
|
Release date:
|
04-Nov-08
|
|
|
|
|
|
|
PROCHECK
|
|
|
|
|
|
Headers
|
|
|
|
References
|
|
|
|
|
|
|
|
P01897
(HA1L_MOUSE) -
H-2 class I histocompatibility antigen, L-D alpha chain from Mus musculus
|
|
|
|
Seq:
Struc:
|
|
|
|
362 a.a.
175 a.a.*
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
|
|
|
|
|
|
| |
|
|
J Immunol
181:6255-6264
(2008)
|
|
PubMed id:
|
|
|
|
|
|
| |
|
Distinct CDR3 conformations in TCRs determine the level of cross-reactivity for diverse antigens, but not the docking orientation.
|
|
L.L.Jones,
L.A.Colf,
J.D.Stone,
K.C.Garcia,
D.M.Kranz.
|
|
|
|
|
| |
ABSTRACT
|
|
|
|
| |
|
|
T cells are known to cross-react with diverse peptide MHC Ags through their
alphabeta TCR. To explore the basis of such cross-reactivity, we examined the 2C
TCR that recognizes two structurally distinct ligands, SIY-K(b) and alloantigen
QL9-L(d). In this study we characterized the cross-reactivity of several
high-affinity 2C TCR variants that contained mutations only in the CDR3alpha
loop. Two of the TCR lost their ability to cross-react with the reciprocal
ligand (SIY-K(b)), whereas another TCR (m67) maintained reactivity with both
ligands. Crystal structures of four of the TCRs in complex with QL9-L(d) showed
that CDR1, CDR2, and CDR3beta conformations and docking orientations were
remarkably similar. Although the CDR3alpha loop of TCR m67 conferred a 2000-fold
higher affinity for SIY-K(b), the TCR maintained the same docking angle on
QL9-L(d) as the 2C TCR. Thus, CDR3alpha dictated the affinity and level of
cross-reactivity, yet it did so without affecting the conserved docking
orientation.
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
 |
 |
 |
|
Literature references that cite this PDB file's key reference
|
|
|
| |
PubMed id
|
|
Reference
|
|
|
|
|
|
R.L.Rich,
and
D.G.Myszka
(2010).
Grading the commercial optical biosensor literature-Class of 2008: 'The Mighty Binders'.
|
| |
J Mol Recognit,
23,
1.
|
|
|
|
|
|
|
A.S.Chervin,
J.D.Stone,
P.D.Holler,
A.Bai,
J.Chen,
H.N.Eisen,
and
D.M.Kranz
(2009).
The impact of TCR-binding properties and antigen presentation format on T cell responsiveness.
|
| |
J Immunol,
183,
1166-1178.
|
|
|
|
|
|
|
J.D.Stone,
A.S.Chervin,
and
D.M.Kranz
(2009).
T-cell receptor binding affinities and kinetics: impact on T-cell activity and specificity.
|
| |
Immunology,
126,
165-176.
|
|
|
|
|
|
|
K.C.Garcia,
J.J.Adams,
D.Feng,
and
L.K.Ely
(2009).
The molecular basis of TCR germline bias for MHC is surprisingly simple.
|
| |
Nat Immunol,
10,
143-147.
|
|
|
|
|
|
|
K.Rubtsova,
J.P.Scott-Browne,
F.Crawford,
S.Dai,
P.Marrack,
and
J.W.Kappler
(2009).
Many different Vbeta CDR3s can reveal the inherent MHC reactivity of germline-encoded TCR V regions.
|
| |
Proc Natl Acad Sci U S A,
106,
7951-7956.
|
|
|
|
|
|
|
N.A.Bowerman,
L.A.Colf,
K.C.Garcia,
and
D.M.Kranz
(2009).
Different strategies adopted by K(b) and L(d) to generate T cell specificity directed against their respective bound peptides.
|
| |
J Biol Chem,
284,
32551-32561.
|
|
|
|
|
|
|
S.J.Turner,
N.L.La Gruta,
K.Kedzierska,
P.G.Thomas,
and
P.C.Doherty
(2009).
Functional implications of T cell receptor diversity.
|
| |
Curr Opin Immunol,
21,
286-290.
|
|
|
|
|
|
|
L.L.Jones,
L.A.Colf,
A.J.Bankovich,
J.D.Stone,
Y.G.Gao,
C.M.Chan,
R.H.Huang,
K.C.Garcia,
and
D.M.Kranz
(2008).
Different thermodynamic binding mechanisms and peptide fine specificities associated with a panel of structurally similar high-affinity T cell receptors.
|
| |
Biochemistry,
47,
12398-12408.
|
|
|
PDB code:
|
|
|
|
|
|
|
The most recent references are shown first.
Citation data come partly from CiteXplore and partly
from an automated harvesting procedure. Note that this is likely to be
only a partial list as not all journals are covered by
either method. However, we are continually building up the citation data
so more and more references will be included with time.
Where a reference describes a PDB structure, the PDB
code is
shown on the right.
|
|
| |
Links
