PDBsum entry: 3dq0

Oxidoreductase

PDB id: 3dq0

Contents

Protein chain

498 a.a. *

Ligands

NAG ×3

NAG-NAG ×2

FAD

MZO

Waters ×365

* Residue conservation analysis

PDB id:

3dq0

Name:

Oxidoreductase

Title:

Maize cytokinin oxidase/dehydrogenase complexed with n6-(3-m phenyl)adenine

Structure:

Cytokinin dehydrogenase 1. Chain: a. Synonym: cytokinin oxidase 1, cko 1, cox 1, zmckx1. Engineered: yes

Source:

Zea mays. Maize. Organism_taxid: 4577. Strain: cultivar nobilis. Gene: ckx1. Expressed in: yarrowia lipolytica. Expression_system_taxid: 4952.

Resolution:

1.90Å R-factor: 0.207 R-free: 0.237

Authors:

D.Kopecny,P.Briozzo

Key ref:

L.Spichal et al. Characterization and biological activity of novel purine-Derived inhibitor of cytokinin oxidase/dehydrogenase and its potential use for in vivo studies. To be published,

Date:

09-Jul-08 Release date: 14-Jul-09

Protein chain

Q9T0N8  (CKX1_MAIZE) -  Cytokinin dehydrogenase 1

Seq: 534 a.a.

Struc: 498 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.1.5.99.12 - Cytokinin dehydrogenase.
• Reaction: N₆-dimethylallyladenine + acceptor + H₂O = adenine + 3-methylbut-2- enal + reduced acceptor

N(6)-dimethylallyladenine (Bound ligand (Het Group name = MZO) matches with 73.68% similarity) + acceptor + H(2)O = adenine + 3-methylbut-2- enal + reduced acceptor

• Cofactor: FAD

FAD (Bound ligand (Het Group name = FAD) corresponds exactly)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Cellular component: extracellular region (2 terms)
• Biological process: oxidation-reduction process (2 terms)
• Biochemical function: catalytic activity (6 terms)