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Cytokine/cytokine receptor PDB id
3dlq
Jmol
Contents
Protein chains
131 a.a. *
201 a.a. *
Waters ×309
* Residue conservation analysis
PDB id:
3dlq
Name: Cytokine/cytokine receptor
Title: Crystal structure of the il-22/il-22r1 complex
Structure: Interleukin-22. Chain: i. Fragment: cytokine, unp residues 29-179. Synonym: il-22, il-10-related t-cell-derived-inducible factor, il-tif. Engineered: yes. Mutation: yes. Interleukin-22 receptor subunit alpha-1. Chain: r.
Source: Homo sapiens. Human. Organism_taxid: 9606. Gene: il22. Expressed in: escherichia coli. Expression_system_taxid: 562. Gene: il22ra1.
Resolution:
1.90Å     R-factor:   0.195     R-free:   0.236
Authors: L.Bleicher,P.R.De Moura,L.Watanabe,D.Colau,L.Dumoutier,J.- C.Renauld,I.Polikarpov
Key ref: L.Bleicher et al. (2008). Crystal structure of the IL-22/IL-22R1 complex and its implications for the IL-22 signaling mechanism. Febs Lett, 582, 2985-2992. PubMed id: 18675809 DOI: 10.1016/j.febslet.2008.07.046
Date:
28-Jun-08     Release date:   19-Aug-08    
PROCHECK
Go to PROCHECK summary
 Headers
 References

Protein chain
Pfam   ArchSchema ?
Q9GZX6  (IL22_HUMAN) -  Interleukin-22
Seq:
Struc: 		179 a.a.
131 a.a.*
Protein chain
Pfam   ArchSchema ?
Q8N6P7  (I22R1_HUMAN) -  Interleukin-22 receptor subunit alpha-1
Seq:
Struc: 		 
Seq:
Struc: 		574 a.a.
201 a.a.*
Key:    PfamA domain  PfamB domain  Secondary structure  CATH domain
* PDB and UniProt seqs differ at 2 residue positions (black crosses)

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     extracellular region   2 terms 
  Biological process     blood coagulation   1 term 

 

 
DOI no: 10.1016/j.febslet.2008.07.046 Febs Lett 582:2985-2992 (2008)
PubMed id: 18675809  
 
 
Crystal structure of the IL-22/IL-22R1 complex and its implications for the IL-22 signaling mechanism.
L.Bleicher, P.R.de Moura, L.Watanabe, D.Colau, L.Dumoutier, J.C.Renauld, I.Polikarpov.
 
  ABSTRACT  
 
Interleukin-22 (IL-22) is a member of the interleukin-10 cytokine family, which is involved in anti-microbial defenses, tissue damage protection and repair, and acute phase responses. Its signaling mechanism involves the sequential binding of IL-22 to interleukin-22 receptor 1 (IL-22R1), and of this dimer to interleukin-10 receptor 2 (IL-10R2) extracellular domain. We report a 1.9A crystal structure of the IL-22/IL-22R1 complex, revealing crucial interacting residues at the IL-22/IL-22R1 interface. Functional importance of key residues was confirmed by site-directed mutagenesis and functional studies. Based on the X-ray structure of the binary complex, we discuss a molecular basis of the IL-22/IL-22R1 recognition by IL-10R2. STRUCTURED SUMMARY:
 

Literature references that cite this PDB file's key reference

  PubMed id Reference
21166540 W.Ouyang, S.Rutz, N.K.Crellin, P.A.Valdez, and S.G.Hymowitz (2011).
Regulation and functions of the IL-10 family of cytokines in inflammation and disease.
  Annu Rev Immunol, 29, 71.  
20846897 A.Zdanov (2010).
Structural analysis of cytokines comprising the IL-10 family.
  Cytokine Growth Factor Rev, 21, 325-330.  
20454917 D.B.Trivella, J.R.Ferreira-Júnior, L.Dumoutier, J.C.Renauld, and I.Polikarpov (2010).
Structure and function of interleukin-22 and other members of the interleukin-10 family.
  Cell Mol Life Sci, 67, 2909-2935.  
20712455 G.Gallagher, N.J.Megjugorac, R.Y.Yu, J.Eskdale, G.E.Gallagher, R.Siegel, and E.Tollar (2010).
The lambda interferons: guardians of the immune-epithelial interface and the T-helper 2 response.
  J Interferon Cytokine Res, 30, 603-615.  
20712454 H.H.Gad, O.J.Hamming, and R.Hartmann (2010).
The structure of human interferon lambda and what it has taught us.
  J Interferon Cytokine Res, 30, 565-571.  
20127093 K.Wolk, E.Witte, K.Witte, K.Warszawska, and R.Sabat (2010).
Biology of interleukin-22.
  Semin Immunopathol, 32, 17-31.  
20947410 R.P.Donnelly, F.Sheikh, H.Dickensheets, R.Savan, H.A.Young, and M.R.Walter (2010).
Interleukin-26: an IL-10-related cytokine produced by Th17 cells.
  Cytokine Growth Factor Rev, 21, 393-401.  
20462497 S.I.Yoon, B.C.Jones, N.J.Logsdon, B.D.Harris, A.Deshpande, S.Radaeva, B.A.Halloran, B.Gao, and M.R.Walter (2010).
Structure and mechanism of receptor sharing by the IL-10R2 common chain.
  Structure, 18, 638-648.
PDB code: 3lqm
  20671117 S.Meng, Q.Gui, Q.Xu, K.Lu, X.Jiao, J.Fan, B.Ge, Y.Ke, S.Zhang, J.Wu, and C.Wang (2010).
Association of Shp2 with phosphorylated IL-22R1 is required for interleukin-22-induced MAP kinase activation.
  J Mol Cell Biol, 2, 223-230.  
21106435 W.Ouyang (2010).
Distinct roles of IL-22 in human psoriasis and inflammatory bowel disease.
  Cytokine Growth Factor Rev, 21, 435-441.  
19457860 H.H.Gad, C.Dellgren, O.J.Hamming, S.Vends, S.R.Paludan, and R.Hartmann (2009).
Interferon-lambda is functionally an interferon but structurally related to the interleukin-10 family.
  J Biol Chem, 284, 20869-20875.
PDB code: 3hhc
  19193995 L.Watanabe, P.R.de Moura, A.S.Nascimento, D.Colau, L.Dumoutier, J.C.Renauld, and I.Polikarpov (2009).
Crystallization and preliminary X-ray diffraction analysis of human IL-22 bound to its soluble decoy receptor IL-22BP.
  Acta Crystallogr Sect F Struct Biol Cryst Commun, 65, 102-104.  
The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time. Where a reference describes a PDB structure, the PDB code is shown on the right.