PDBsum entry 3de3

Hydrolase

PDB id

3de3

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Contents

			Protein chain

					279 a.a. *

			Metals

					_CA

			Waters ×198

* Residue conservation analysis

PDB id:

3de3

Links
- PDBe
- RCSB
- MMDB
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- Proteopedia
- CATH
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- PDBSWS
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Name:

Hydrolase

Title:

Proteinase k by classical hanging drop method before high x-ray dose on esrf id23-1 beamline

Structure:

Proteinase k. Chain: x. Synonym: tritirachium alkaline proteinase, endopeptidase k. Ec: 3.4.21.64

Source:

Engyodontium album. Organism_taxid: 37998. Strain: limber

Resolution:

1.43Å

R-factor:

0.206

R-free:

0.229

Authors:

E.Pechkova,S.K.Tripathi,C.Nicolini

Key ref:

E.Pechkova et al. (2009). Radiation stability of proteinase K crystals grown by LB nanotemplate method. J Struct Biol, 168, 409-418. PubMed id: 19686853

Date:

08-Jun-08

Release date:

09-Jun-09

PROCHECK

	Headers

	References

Protein chain

P06873 (PRTK_PARAQ) - Proteinase K from Parengyodontium album

Seq: Struc:					384 a.a. 279 a.a.

Key:

PfamA domain

Secondary structure

CATH domain



		Enzyme reactions

Enzyme class:

E.C.3.4.21.64 - peptidase K.

[IntEnz] [ExPASy] [KEGG] [BRENDA]

Reaction:

Hydrolysis of keratin and of other proteins, with subtilisin-like specificity. Hydrolyzes peptides amides.

	J Struct Biol 168:409-418 (2009)
PubMed id: 19686853

Radiation stability of proteinase K crystals grown by LB nanotemplate method.
E.Pechkova, S.Tripathi, R.B.Ravelli, S.McSweeney, C.Nicolini.

ABSTRACT

A detailed analysis of structural and intensity changes induced by X-ray radiation is presented for two types of proteinase K crystals: crystal grown by classical hanging drop method and those grown by Langmuir-Blodgett (LB) nanotemplate. The comparison of various parameters (e.g. intensity per sigma ratio, unit-cell volume, number of unique reflections, B-factors) and electron density maps as a function of radiation dose, demonstrates that crystals, grown by the LB nanotemplate method, appear to be more resistant against radiation damage than crystals grown by the classical hanging drop method.

Literature references that cite this PDB file's key reference

PubMed id

Reference

21335918

E.Pechkova, and C.Nicolini (2011).
In situ study of nanotemplate-induced growth of lysozyme microcrystals by submicrometer GISAXS.

J Synchrotron Radiat, 18, 287-292.

21525646

R.M.Leal, G.P.Bourenkov, O.Svensson, D.Spruce, M.Guijarro, and A.N.Popov (2011).
Experimental procedure for the characterization of radiation damage in macromolecular crystals.

J Synchrotron Radiat, 18, 381-386.

20662638

C.Nicolini, and E.Pechkova (2010).
Nanoproteomics for nanomedicine.

Nanomedicine (Lond), 5, 677-682.

The most recent references are shown first. Citation data come partly from CiteXplore and partly from an automated harvesting procedure. Note that this is likely to be only a partial list as not all journals are covered by either method. However, we are continually building up the citation data so more and more references will be included with time.