PDBsum entry: 3ci6

Transferase

PDB id: 3ci6

Contents

Protein chains

158 a.a. *

166 a.a. *

Ligands

P4G ×2

PEG ×2

GOL ×2

Waters ×285

* Residue conservation analysis

PDB id:

3ci6

Name:

Transferase

Title:

Crystal structure of the gaf domain from acinetobacter phosphoenolpyruvate-protein phosphotransferase

Structure:

Phosphoenolpyruvate-protein phosphotransferase. Chain: a, b. Fragment: gaf domain: residues 1-168. Engineered: yes

Source:

Acinetobacter sp.. Organism_taxid: 62977. Strain: adp1. Gene: ptsp, aciad0454. Expressed in: escherichia coli bl21(de3). Expression_system_taxid: 469008.

Resolution:

1.55Å R-factor: 0.170 R-free: 0.196

Authors:

M.E.Cuff,G.Shackelford,Y.Kim,A.Joachimiak,Midwest Center For Structural Genomics (Mcsg)

Key ref:

M.E.Cuff et al. Crystal structure of the gaf domain from acinetobacter phosphoenolpyruvate-Protein phosphotransferase.. To be published,

Date:

10-Mar-08 Release date: 13-May-08

Protein chain

Q6FEW8  (Q6FEW8_ACIAD) -  Phosphoenolpyruvate-protein phosphotransferase

Seq: 764 a.a.

Struc: 158 a.a.

Protein chain

Q6FEW8  (Q6FEW8_ACIAD) -  Phosphoenolpyruvate-protein phosphotransferase

Seq: 764 a.a.

Struc: 166 a.a.

Enzyme reactions

• Enzyme class: Chains A, B: E.C.2.7.3.9 - Phosphoenolpyruvate--protein phosphotransferase.
• Reaction: Phosphoenolpyruvate + protein L-histidine = pyruvate + protein N(pi)- phospho-L-histidine

Phosphoenolpyruvate + protein L-histidine = pyruvate (Bound ligand (Het Group name = GOL) matches with 71.43% similarity) + protein N(pi)- phospho-L-histidine

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Biochemical function: protein binding (1 term)