PDBsum entry: 3cfp

Transferase/DNA

PDB id: 3cfp

Contents

Protein chain

901 a.a. *

DNA/RNA

Ligands

TTP

Metals

_CL

_CA ×4

Waters ×299

* Residue conservation analysis

PDB id:

3cfp

Name:

Transferase/DNA

Title:

Structure of the replicating complex of a pol alpha family d polymerase, ternary complex 1

Structure:

DNA polymerase. Chain: a. Synonym: gp43. Engineered: yes. Mutation: yes. DNA (5'- d( Dap Dcp Dap Dgp Dgp Dtp Dap Dap Dgp Dcp Dap Dgp Dtp Dcp P Dg)-3'). Chain: t.

Source:

Bacteriophage rb69. Gene: 43. Expressed in: escherichia coli. Expression_system_taxid: 562. Synthetic: yes. Other_details: synthesized by w.M. Keck facility at yale university). Other_details: synthesized by w.M. Keck facility at yale un

Resolution:

2.50Å R-factor: 0.200 R-free: 0.267

Authors:

J.Wang,D.Klimenko,M.Wang,T.A.Steitz,W.H.Konigsberg

Key ref:

D.Klimenko et al. To be published,

Date:

04-Mar-08 Release date: 10-Mar-09

Protein chain

Q38087  (DPOL_BPR69) -  DNA polymerase

Seq: 903 a.a.

Struc: 901 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

Deoxynucleoside triphosphate (Bound ligand (Het Group name = TTP) matches with 66.67% similarity) + DNA(n) = diphosphate + DNA(n+1)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Biological process: nucleobase, nucleoside, nucleotide and nucleic acid metabolic process (2 terms)
• Biochemical function: nucleotide binding (9 terms)