PDBsum entry: 3cfo

Transferase

PDB id: 3cfo

Contents

Protein chain

906 a.a. *

Ligands

SO4 ×4

GMP

Waters ×257

* Residue conservation analysis

PDB id:

3cfo

Name:

Transferase

Title:

Triple mutant apo structure

Structure:

DNA polymerase. Chain: a. Synonym: gp43. Engineered: yes. Mutation: yes

Source:

Bacteriophage rb69. Gene: 43. Expressed in: escherichia coli. Expression_system_taxid: 562

Resolution:

2.60Å R-factor: 0.194 R-free: 0.235

Authors:

J.Wang,D.Klimenko,M.Wang,T.A.Steitz,W.H.Konigsberg

Key ref:

D.Klimenko et al. Insights into base selectivity from the structures of an rb69 DNA polymerase triple mutant. To be published,

Date:

04-Mar-08 Release date: 10-Mar-09

Protein chain

Q38087  (DPOL_BPR69) -  DNA polymerase

Seq: 903 a.a.

Struc: 906 a.a.*

* PDB and UniProt seqs differ at 3 residue positions (black crosses)

Enzyme reactions

• Enzyme class: E.C.2.7.7.7 - DNA-directed Dna polymerase.
• Reaction: Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

Molecule diagrams generated from .mol files obtained from the KEGG ftp site

 Gene Ontology (GO) functional annotation 

• Biological process: nucleobase, nucleoside, nucleotide and nucleic acid metabolic process (2 terms)
• Biochemical function: nucleotide binding (9 terms)