PDBsum entry 3c9k

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protein Protein-protein interface(s) links
DNA binding protein PDB id
Protein chains
101 a.a. *
90 a.a. *
93 a.a. *
78 a.a. *
* Residue conservation analysis
PDB id:
Name: DNA binding protein
Title: Model of histone octamer tubular crystals
Structure: Histone h2a-iv. Chain: a, e. Histone h2b 7. Chain: b, f. Synonym: h2b vii. Histone h3.2. Chain: c, g. Histone h4. Chain: d, h
Source: Gallus gallus. Chicken. Organism_taxid: 9031. Tissue: blood, erythrocytes. Tissue: blood, erythrocytes
Authors: T.D.Frouws
Key ref: T.D.Frouws et al. (2009). Histone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber. Biophys J, 96, 3363-3371. PubMed id: 19383479
16-Feb-08     Release date:   06-Jan-09    
Go to PROCHECK summary

Protein chains
Pfam   ArchSchema ?
P02263  (H2A4_CHICK) -  Histone H2A-IV
129 a.a.
101 a.a.
Protein chains
Pfam   ArchSchema ?
P0C1H5  (H2B7_CHICK) -  Histone H2B 7
126 a.a.
90 a.a.
Protein chains
Pfam   ArchSchema ?
P84229  (H32_CHICK) -  Histone H3.2
136 a.a.
93 a.a.
Protein chains
Pfam   ArchSchema ?
P62801  (H4_CHICK) -  Histone H4
103 a.a.
78 a.a.
Key:    PfamA domain  Secondary structure

 Gene Ontology (GO) functional annotation 
  GO annot!
  Cellular component     protein complex   9 terms 
  Biological process     nucleosome assembly   4 terms 
  Biochemical function     DNA binding     3 terms  


Biophys J 96:3363-3371 (2009)
PubMed id: 19383479  
Histone octamer helical tubes suggest that an internucleosomal four-helix bundle stabilizes the chromatin fiber.
T.D.Frouws, H.G.Patterton, B.T.Sewell.
A major question in chromatin involves the exact organization of nucleosomes within the 30-nm chromatin fiber and its structural determinants of assembly. Here we investigate the structure of histone octamer helical tubes via the method of iterative helical real-space reconstruction. Accurate placement of the x-ray structure of the histone octamer within the reconstructed density yields a pseudoatomic model for the entire helix, and allows precise identification of molecular interactions between neighboring octamers. One such interaction that would not be obscured by DNA in the nucleosome consists of a twofold symmetric four-helix bundle formed between pairs of H2B-alpha3 and H2B-alphaC helices of neighboring octamers. We believe that this interface can act as an internucleosomal four-helix bundle within the context of the chromatin fiber. The potential relevance of this interface in the folding of the 30-nm chromatin fiber is discussed.

Literature references that cite this PDB file's key reference

  PubMed id Reference
19346124 A.Bassett, S.Cooper, C.Wu, and A.Travers (2009).
The folding and unfolding of eukaryotic chromatin.
  Curr Opin Genet Dev, 19, 159-165.  
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